• 靶点-Target： E-cadherin
• MW(Observed)： 125-130kD
• 宿主物种-Host Species： Mouse
• 修饰-Modification： Unmodified
• 推荐稀释比： WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： Affinity purification
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 克隆性： Monoclonal
• 免疫原： Purified recombinant fragment of human E-cadherin expressed in E. Coli.
• 特异性： E-cadherin Monoclonal Antibody detects endogenous levels of E-cadherin protein.
• 基因名称： CDH1
• 蛋白名称： Cadherin-1
• Organism-1： Human
• 基因ID-1： 999
• SwissProt-1： P12830
• Organism-2： Mouse
• 基因ID-2： 12550
• SwissProt-2： P09803
• 背景： This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants， at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats， a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric， breast， colorectal， thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation， invasion， and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq， Nov 2015]，
• 细胞定位： Cell junction， adherens junction . Cell membrane ; Single-pass type I membrane protein. Endosome. Golgi apparatus， trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-， beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx， results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

资料下载