- 商品货号:FH1759
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pECMV-3×FLAG-SCNN1A(NM_001038.5)是一个人源基因哺乳细胞过表达质粒。
- 质粒图谱1: png/1-1G219152301X9.png
质粒序列: 点击查看序列LOCUS Exported 7495 bp ds-DNA circular SYN 24-NOV-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..7495/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 235..614/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 615..818/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 863..881/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"regulatory 913..922/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"CDS 922..987/codon_start=1/product="three tandem FLAG(R) epitope tags, followed by anenterokinase cleavage site"/label=3xFLAG/translation="DYKDHDGDYKDHDIDYKDDDDK"CDS 1009..3018/codon_start=1/label=SCNN1A/translation="MEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP"polyA_signal 3095..3319/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 3365..3793/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 3807..4136/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 3987..4122/label=SV40 ori/note="SV40 origin of replication"CDS 4203..4997/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 5171..5292/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(5743..6328)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(6499..7359)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(7360..7464)/gene="bla"/label=AmpR promoterORIGIN1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccat ggaggggaac1021 aagctggagg agcaggactc tagccctcca cagtccactc cagggctcat gaaggggaac1081 aagcgtgagg agcaggggct gggccccgaa cctgcggcgc cccagcagcc cacggcggag1141 gaggaggccc tgatcgagtt ccaccgctcc taccgagagc tcttcgagtt cttctgcaac1201 aacaccacca tccacggcgc catccgcctg gtgtgctccc agcacaaccg catgaagacg1261 gccttctggg cagtgctgtg gctctgcacc tttggcatga tgtactggca attcggcctg1321 cttttcggag agtacttcag ctaccccgtc agcctcaaca tcaacctcaa ctcggacaag1381 ctcgtcttcc ccgcagtgac catctgcacc ctcaatccct acaggtaccc ggaaattaaa1441 gaggagctgg aggagctgga ccgcatcaca gagcagacgc tctttgacct gtacaaatac1501 agctccttca ccactctcgt ggccggctcc cgcagccgtc gcgacctgcg ggggactctg1561 ccgcacccct tgcagcgcct gagggtcccg cccccgcctc acggggcccg tcgagcccgt1621 agcgtggcct ccagcttgcg ggacaacaac ccccaggtgg actggaagga ctggaagatc1681 ggcttccagc tgtgcaacca gaacaaatcg gactgcttct accagacata ctcatcaggg1741 gtggatgcgg tgagggagtg gtaccgcttc cactacatca acatcctgtc gaggctgcca1801 gagactctgc catccctgga ggaggacacg ctgggcaact tcatcttcgc ctgccgcttc1861 aaccaggtct cctgcaacca ggcgaattac tctcacttcc accacccgat gtatggaaac1921 tgctatactt tcaatgacaa gaacaactcc aacctctgga tgtcttccat gcctggaatc1981 aacaacggtc tgtccctgat gctgcgcgca gagcagaatg acttcattcc cctgctgtcc2041 acagtgactg gggcccgggt aatggtgcac gggcaggatg aacctgcctt tatggatgat2101 ggtggcttta acttgcggcc tggcgtggag acctccatca gcatgaggaa ggaaaccctg2161 gacagacttg ggggcgatta tggcgactgc accaagaatg gcagtgatgt tcctgttgag2221 aacctttacc cttcaaagta cacacagcag gtgtgtattc actcctgctt ccaggagagc2281 atgatcaagg agtgtggctg tgcctacatc ttctatccgc ggccccagaa cgtggagtac2341 tgtgactaca gaaagcacag ttcctggggg tactgctact ataagctcca ggttgacttc2401 tcctcagacc acctgggctg tttcaccaag tgccggaagc catgcagcgt gaccagctac2461 cagctctctg ctggttactc acgatggccc tcggtgacat cccaggaatg ggtcttccag2521 atgctatcgc gacagaacaa ttacaccgtc aacaacaaga gaaatggagt ggccaaagtc2581 aacatcttct tcaaggagct gaactacaaa accaattctg agtctccctc tgtcacgatg2641 gtcaccctcc tgtccaacct gggcagccag tggagcctgt ggttcggctc ctcggtgttg2701 tctgtggtgg agatggctga gctcgtcttt gacctgctgg tcatcatgtt cctcatgctg2761 ctccgaaggt tccgaagccg atactggtct ccaggccgag ggggcagggg tgctcaggag2821 gtagcctcca ccctggcatc ctcccctcct tcccacttct gcccccaccc catgtctctg2881 tccttgtccc agccaggccc tgctccctct ccagccttga cagcccctcc ccctgcctat2941 gccaccctgg gcccccgccc atctccaggg ggctctgcag gggccagttc ctccacctgt3001 cctctggggg ggccctgaga attctgcaga tatccagcac agtggcggcc gctcgagtct3061 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct3121 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt3181 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg3241 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg3301 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat3361 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg3421 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc3481 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga3541 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt服务时间:24小时在线客服
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