- 商品货号:FH1728
- 规格:10μl质粒
- 启动子:CMV
- 复制子:pUC
- 终止子:bGH
- 原核抗性:氨苄青霉素Amp
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pCDNA3.1-EEF2-Myc-6×His(NM_001961.3)是一个人源基因哺乳细胞过表达质粒。
- 质粒图谱1: png/1-1G213160513137.png
质粒序列: 点击查看序列LOCUS Exported 8085 bp ds-DNA circular SYN 13-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..8085/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 235..614/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 615..818/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 863..881/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"regulatory 960..969/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"misc_feature 966..3539/label=EEF2(NM_001961.3)CDS 3570..3599/codon_start=1/product="Myc (human c-Myc proto-oncogene) epitope tag"/label=Myc/translation="EQKLISEEDL"CDS 3615..3632/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 3682..3906/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 3952..4380/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 4394..4723/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 4574..4709/label=SV40 ori/note="SV40 origin of replication"CDS 4790..5584/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 5758..5879/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(6330..6918)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(7089..7949)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(7950..8054)/gene="bla"/label=AmpR promoterORIGIN1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcg961 ccaccatggt gaacttcacg gtagaccaga tccgcgccat catggacaag aaggccaaca1021 tccgcaacat gtctgtcatc gcccacgtgg accatggcaa gtccacgctg acagactccc1081 tggtgtgcaa ggcgggcatc atcgcctcgg cccgggccgg ggagacacgc ttcactgata1141 cccggaagga cgagcaggag cgttgcatca ccatcaagtc aactgccatc tccctcttct1201 acgagctctc ggagaatgac ttgaacttca tcaagcagag caaggacggt gccggcttcc1261 tcatcaacct cattgactcc cccgggcatg tcgacttctc ctcggaggtg actgctgccc1321 tccgagtcac cgatggcgca ttggtggtgg tggactgcgt gtcaggcgtg tgcgtgcaga1381 cggagacagt gctgcggcag gccattgccg agcgcatcaa gcctgtgctg atgatgaaca1441 agatggaccg cgccctgctg gagctgcagc tggagcccga ggagctctac cagactttcc1501 agcgcatcgt ggagaacgtg aacgtcatca tctccaccta cggcgagggc gagagcggcc1561 ccatgggcaa catcatgatc gatcctgtcc tcggtaccgt gggctttggg tctggcctcc1621 acgggtgggc cttcaccctg aagcagtttg ccgagatgta tgtggccaag ttcgccgcca1681 agggggaggg ccagttgggg cctgccgagc gggccaagaa agtagaggac atgatgaaga1741 agctgtgggg tgacaggtac tttgacccag ccaacggcaa gttcagcaag tcagccacca1801 gccccgaagg gaagaagctg ccacgcacct tctgccagct gatcctggac cccatcttca1861 aggtgtttga tgcgatcatg aatttcaaga aagaggagac agcaaaactg atagagaaac1921 tggacatcaa actggacagc gaggacaagg acaaagaagg caaacccctg ctgaaggctg1981 tgatgcgccg ctggctgcct gccggagacg ccttgttgca gatgatcacc atccacctgc2041 cctcccctgt gacggcccag aagtaccgct gcgagctcct gtacgagggg cccccggacg2101 acgaggctgc catgggcatt aaaagctgtg accccaaagg ccctcttatg atgtatattt2161 ccaaaatggt gccaacctcc gacaaaggtc ggttctacgc ctttggacga gtcttctcgg2221 ggctggtctc cactggcctg aaggtcagga tcatggggcc caactatacc cctgggaaga2281 aggaggacct ctacctgaag ccaatccaga gaacaatctt gatgatgggc cgctacgtgg2341 agcccatcga ggatgtgcct tgtgggaaca ttgtgggcct cgtgggcgtg gaccagttcc2401 tggtgaagac gggcaccatc accaccttcg agcacgcgca caacatgcgg gtgatgaagt2461 tcagcgtcag ccctgttgtc agagtggccg tggaggccaa gaacccggct gacctgccca2521 agctggtgga ggggctgaag cggctggcca agtccgaccc catggtgcag tgcatcatcg2581 aggagtcggg agagcatatc atcgcgggcg ccggcgagct gcacctggag atctgcctga2641 aggacctgga ggaggaccac gcctgcatcc ccatcaagaa atctgacccg gtcgtctcgt2701 accgcgagac ggtcagtgaa gagtcgaacg tgctctgcct ctccaagtcc cccaacaagc2761 acaaccggct gtacatgaag gcgcggccct tccccgacgg cctggccgag gacatcgata2821 aaggcgaggt gtccgcccgt caggagctca agcagcgggc gcgctacctg gccgagaagt2881 acgagtggga cgtggctgag gcccgcaaga tctggtgctt tgggcccgac ggcaccggcc2941 ccaacatcct caccgacatc accaagggtg tgcagtacct caacgagatc aaggacagtg3001 tggtggccgg cttccagtgg gccaccaagg agggcgcact gtgtgaggag aacatgcggg3061 gtgtgcgctt cgacgtccac gacgtcaccc tgcacgccga cgccatccac cgcggagggg3121 gccagatcat ccccacagca cggcgctgcc tctatgccag tgtgctgacc gcccagccac3181 gcctcatgga gcccatctac cttgtggaga tccagtgtcc agagcaggtg gtcggtggca3241 tctacggggt tttgaacagg aagcggggcc acgtgttcga ggagtcccag gtggccggca3301 cccccatgtt tgtggtcaag gcctatctgc ccgtcaacga gtcctttggc ttcaccgctg3361 acctgaggtc caacacgggc ggccaggcgt tcccccagtg tgtgtttgac cactggcaga3421 tcctgcccgg agaccccttc gacaacagca gccgccccag ccaggtggtg gcggagaccc3481 gcaagcgcaa gggcctgaaa gaaggcatcc ctgccctgga caacttcctg gacaaattgg3541 gatccgagct cggtaccaag cttgggcccg aacaaaaact catctcagaa gaggatctga3601 atagcgccgt cgaccatcat catcatcatc attgagttta aacggtctcc agcttaagtt3661 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct3721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg3781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc3841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct3901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct3961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg4021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg4081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac4141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct4201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt4261 tccaaactgg aacaacactc aaccctatct cggtctat
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