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- 规格：10μl质粒
- 复制子：ori
- 终止子：rrnB T1 terminator
- 质粒分类：基因文库质粒；其它基因质粒；其它种源质粒
- 质粒大小：7794bp
- 原核抗性：卡那霉素Kan（50μg/ml）
- 克隆菌株：DH5α等大肠杆菌
- 质粒简介： pJV62质粒提供分枝杆菌噬菌体的Che9c gene 61基因，本质粒的参考文献：Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets.
- 质粒图谱1： png/1-1FQ51J119612.png
质粒序列： 点击查看序列LOCUS Exported 7794 bp ds-DNA circular SYN 15-8-2017KEYWORDS pJV62FEATURES Location/Qualifierssource 1..7794/organism="synthetic DNA construct"/mol_type="other DNA"CDS 4574..5635/codon_start=1/product="Gene/Insert name Che9c gene 61Species MycobacteriophageInsert Size (bp) 1061"/note="Che9c"/translation="MAENAVTKQDSPKAPETISQVLQVLVPQLARAVPKGMDPDRIARIVQTEIRKSRNAKAAGIAKQSLDDCTQESFAGALLTSAALGLEPGVNGECYLVPYRDTRRGVVECQLIIGYQGIVKLFWQHPRASRIDAQWVGANDEFHYTMGLNPTLKHVKAKGDRGNPVYFYAIVEVTGAEPLWDVFTADEIRELRRGKVGSSGDIKDPQRWMERKTALKQVLKLAPKTTRLDAAIRADDRPGTDLSQSQALALPSTVKPTADYIDGEIAEPHEVDTPPKSSRAQRAQRATAPAPDVQMANPDQLKRLGEIQKAEKYNDADWFKFLADSAGVKATRAADLTFDEAKAVIDMFDGPNA"terminator 5700..5746/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"CDS 5907..6722/codon_start=1/gene="aph(3')-Ia"/product="aminoglycoside phosphotransferase"/note="KanR"/note="confers resistance to kanamycin in bacteria or G418(Geneticin(R)) in eukaryotes"/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"rep_origin 7053..7641/direction=RIGHT/note="ori"/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 aacgcgtgag cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc61 aggcggcagg gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag121 cggcccggaa acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg181 gggtgctcgg ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt241 gcagttgtgg ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca301 ccggtgatta atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag361 ctctggctgc cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa421 agccgcgcgt cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac481 ctgctggtcg tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg541 tcccatccgc tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg601 tgggcactca acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac661 atggcggcgt gcgccgaagg ccttcggcgc gccgtcgatg gcgaccgcag ttactcaggc721 ctcatgacca aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc781 tacacactca gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt841 cagcagacca cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat901 tccgtcaggt tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac961 gtggacggac tcggccgcgc gatctatgcc gagtgccacg cgcgaaacgc cgaatttccg1021 tgcaacgacg tgtgtcccgg accgctaccg gacagcgagg tccgcgccat cgccaacagc1081 atttggcgtt ggatcacaac caagtcgcgc atttgggcgg acgggatcgt ggtctacgag1141 gccacactca gtgcgcgcca gtcggccatc tcgcggaagg gcgcagcagc gcgcacggcg1201 gcgagcacag ttgcgcggcg cgcaaagtcc gcgtcagcca tggaggcatt gctatgagcg1261 acggctacag cgacggctac aaccggcagc cgactgtccg caaaaagcgg cgcgtgaccg1321 ccgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc1381 aggaacgcag cgagtggctc gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc1441 acgacgacga gggccactct tggccgcaaa cggccaaaca tttcgggctg catctggaca1501 ccgttaagcg actcggctat cgggcgagga aagagcgtgc ggcagaacag gaagcggctc1561 aaaaggccca caacgaagcc gacaatccac cgctgttcta acgcaattgg ggagcgggtg1621 tcgcgggggt tccgtggggg gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag1681 acgctagttt tctggtttgg gccatgcctg tctcgttgcg tgtttcgttg cgcccgtttt1741 gaataccagc cagacgagac ggggttctac gaatcttggt cgataccaag ccatttccgc1801 tgaatatcgg ggagctcacc gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc1861 gttgtagtgc ctgtggtggc atccgtggcg cggccgcggt accagatctt taaatctaga1921 taaagaagtg acgcggtctc aagcgtcgag cgtcgccagc gtgtcgagga tgtcgaagtc1981 gtagccgtcg gcgctggcga tgtagacctg ctggtcgaat tgactgtcgc gcatacacat2041 cgggccccgg ggcccgtcga acccgacatc gtgcgcggat gccatcaggt ccggtatctc2101 gggggagtgg gcccgctgga agatggcctc gagcgcaagc agaccctcgt aacaggattc2161 ggccatcgcg ttgagcggtg gcgcgtcggc gccgtagcgg gcgacgtagc tgcccatcag2221 gtccatggca cccgcggtgg ccagtgaact gaagtacgcc gcggcgacat agaggttttc2281 ggtggagccg gcgccgctgg ccagcagcat gttctcctcc atcagcgggc tgaaccgcgc2341 catgcggtcg tgcccgccgg cgcgcgcgaa ctcgcggttg aacaacacgg cgtcctggcc2401 gacgagcagc atcaacacgg cctgcgcccc cgacgcgatg gccttgcgga caggtgcgcg2461 gaaatcgtcg gtgccgtacg ggacgtagat ctcccgtctg agctcgaggt ccagatctcg2521 gcagtacgcg cgggcggccg cggcggaacg gcgcggccag atgtagtcat cgccgaccag2581 gcaccaggac cggatgccga agtggtcgcg cagccaggcg agcgcgggcg cgatctggat2641 ctgcggtgtc tcgcctgtgc agaacacgcc cggtgtgcgt tcaccgccct cgtacaacga2701 ggtgtagacg tacgggatgc ggtcgcggac caccggggag atgcggttgc gcacggccga2761 gatgtgccag ccggtcacgg cgtcgagacc gtgacctcgc aaccggtcgg cgacggtccg2821 ggcgacgtcg tcgccgggcg ctccgccgtc gagcacctcg atggtgacct tgcggccctg2881 caggccgcct cggtcgttga cctccttggc cgcgagctcg gccacggcct cgcacgaagg2941 cgcgaagatt cccgctggcc cttgaagcgg aatcaccagc ccgacgcgga actcaacctc3001 gccgtcctgc actccagatc accgtcgatc ccgtgtagtc tgcgcttcaa agctttctag3061 cagaaataat tcattctgaa cagaccccgc cgtcgacacg aggagacacc caccatggcc3121 gccggacagc agcgccgccc caacctcctg ctgccgttgg tgcgtctgac ccacctcgcg3181 gagtcggcga tcgaacgcgt gctcgcggac tcgtcgctca agatcgagga ctggcgcgtg3241 ctcgacgagt tggccggacg gcgcaccgtg cccatgagcg atctcgcgca ggccacgctg3301 atcacgggtc cgactctcac cagaaccgtc gatcgccttg tgtcgcaagg gatcatctac3361 cggactgccg atctgcatga ccgccggcgg gtgctcgtgg cgttgacccc gcgggggcgg3421 acgctgcgca accgcctggt ggacgcggta gccgaggccg agtgtgcggc ttttgaatcg3481 tgcgggctgg acgtcgacca gttgcgcgaa ctcgtcgaca ccacctcgaa tttgacttcg3541 taaccacccg cgcccggcgc gggcgttcac ccttgacttt tattttcatc tggatatatt3601 tcgggtgaat ggaaaggggt gaccatgccg acctacacat tccgttgttc ccactgcggt3661 cccttcgatc tcacctgcgc gatctccgag cgcgatgcgg cggcgacctg tccggagtgc3721 cggacgccgg cgcgccgggt cttcggttcg gtagggctga cgacattcac cgcgggacat3781 caccgcgcat tcgacgcggc gtccgcgagc gccgaaagtc ccacggtggt gaagtcgatt3841 cccgcaggcg cggaccgccc gcgggccccg cgccgcaatc ccggtctacc gagtctgccg3901 aggtactagc gacatgggtg gcgtcgggct cttctacgtg ggtgcggtgc tcatcatcga3961 cgggctgatg ctgctgggcc gcatcagccc acgaggcgca acaccgctga acttcttcgt4021 cggcggactg caggtggtga cgcctacggt gctgatcctg cagtccggcg gagacgcggc4081 cgtgatcttc gcggcctccg ggctctacct gttcggcttc acctacctgt gggtggccat4141 caacaacgtg accgactggg acggagaagg tctcggatgg ttctcgctgt tcgtcgcgat4201 cgccgcactc ggctactcgt ggcacgcgtt caccgccgag gccgacccgg cgttcggggt4261 gatctggctg ctgtgggcag tgctgtggtt catgctgttc ctgctgctcg gcctggggca4321 cgacgcactg gggcccgccg tcgggttcgt cgcggtggcc gaaggcgtga tcaccgccgc4381 cgtgccggcc ttcctgatcg tgtcgggcaa ctgggaaacc ggcccgctcc ccgccgcggt4441 catcgccgtg atcggttttg ccgcagttgt tctcgcatac cccatcgggc gccgtctcgc4501 agcgccgtca gtcaccaacc ctccaccggc cgcgctcgcg gccaccaccc gataagagaa4561 agggagtcca catatggctg aaaatgctgt caccaagcag gattcgccca aggcacccga4621 gacgatctcg caagtgctgc aggtgctcgt gccgcagctg gcgcgtgcag tgcccaaggg4681 tatggacccc gaccgcatcg cgcggatcgt gcaaaccgag atccgcaagt cgcgcaacgc4741 gaaagctgct ggcatcgcta agcagtccct cgacgactgc acgcaagagt catttgccgg4801 tgcgctgctg acctcggccg cgctcggtct cgagcccggt gtcaacggcg agtgctacct4861 cgtgccctac cgcgacaccc ggcgcggcgt ggtcgagtgc cagctgatca tcggctacca4921 gggcatcgtc aaactgttct ggcagcaccc gcgcgcctcc cggatcgacg cgcagtgggt4981 cggcgcgaac gacgaattcc attacaccat gggcctcaat ccgacgctga aacacgtgaa5041 ggccaagggt gatcggggta atccggtcta cttctacgcg atcgtcgagg tgaccggcgc5101 tgagccgctg tgggacgtgt tcaccgccga cgagatcagg gaattgcgtc gcggcaaggt5161 cggatcctcg ggcgacatca aggacccgca gcgctggatg gagcggaaga ccgcgctcaa5221 acaggtgctg aagctggcac cgaagacgac gcggctcgac gcggcgatcc gcgccgacga5281 tcgcccgggc accgacctgt cacagtcgca ggcgctcgcg ctgccgtcga ccgtcaagcc5341 gacggccgac tacatcgacg gcgagatcgc cgagccgcac gaggtcgata cgccaccgaa5401 gtcgtcgcgc gcacaacgcg cgcagcgcgc caccgcgccg gcgcccgacg tgcagatggc5461 caatcccgat cagctgaagc gcctcggcga gatccagaag gccgaaaagt acaacgacgc5521 cgattggttc aagttcctcg ccgattcggc cggcgtcaaa gccacgcgcg ccgccgacct5581 cacgttcgac gaggcgaagg ctgtcatcga catgttcgac gggcccaacg catgagcgcc5641 ccggcgaatt ccgacgcggt ggttgatctg caactagcgt acgatcgact gccaggcatc5701 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatgcca tcatggccgc5761 ggtgatcagc tagccacctg acgtcggggg ggggggaaag ccacgttgtg tctcaaaatc5821 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt5881 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga5941 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata6001 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt6061 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac6121 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg6181 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag6241 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc6301 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg6361 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg6421 gctggcctgt tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt6481 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa6541 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc6601 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg6661 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct6721 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg6781 gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc6841 cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta
