- 商品货号:FH1173
- 规格:10μl质粒
- 启动子:CMV promoter
- 复制子:Ori,F1 ori
- 终止子:hGH poly(A) signal
- 质粒分类:基因文库质粒;其它基因质粒;猪源基因质粒
- 质粒大小:7884bp
- 质粒标签:N-3×FLAG
- 原核抗性:氨苄青霉素Amp(100μg/ml)
- 筛选标记:新霉素Neo/G418
- 克隆菌株:DH5α等大肠杆菌
- 表达宿主:293T等哺乳细胞
- 质粒简介: p3×FLAG-CMV-10-RIP2-p质粒是一个猪源基因过表达质粒,CMV启动子驱动3×FLAG标签和RIP2基因融合表达。p3×FLAG-CMV-10-RIP2-p质粒由淼灵生物开发。
PREDICTED: Sus scrofa receptor interacting serine/threonine kinase 2 (RIPK2), transcript variant X1, mRNA.NCBI Reference Sequence: XM_021089139.1.- 质粒图谱1: png/1-1FQ6154102R6.png
质粒序列: 点击查看序列LOCUS Exported 7884 bp ds-DNA circular SYN 16-AUG-2017DEFINITION synthetic circular DNAKEYWORDS p3×FLAG-CMV-10-RIP2-p.dnaFEATURES Location/Qualifierssource 1..7884/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 318..697/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 698..901/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"CDS 931..996/codon_start=1/product="three tandem FLAG(R) epitope tags, followed by anenterokinase cleavage site"/label=3xFLAG/translation="DYKDHDGDYKDHDIDYKDDDDK"CDS 1015..2616/codon_start=1/label=RIP2-p/translation="MNEEAICSALPTIPYRKLADLCYLSRGASGTVSSARHADWRIQVAVKHLHIHTPLLDSERNDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKNEYPDVPWPLRFRILHEIALGVNYLHNMNPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSIKSAPEGGTIIYMPPENYEPGQKSRATVKHDIYSYAVITWEVLSRKQPFEEVTNPLQIMYSVSQGHRPDTSEESLPFDIPHRALMISLIESGWAQNPDERPSFLKCLIELEPVLRIFEDITFLEAVIQLKKTKLQSASSTVHLCDKKKTELYPNTSVNHGSQEESCGSSQLHKPSNSPGTSKSLSATQDSDFLSRKTQDFSTPRQCPVNRSWDSNISVAQRATFCDHKTTPCSLAIINPLSAEGNSERFQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELISTKPTRTSKVRQLLDTTDIQGEEFARVIVQKLKDNKQMGLQPYPEILVVSRPPSLNLF"polyA_signal 2643..3265/label=hGH poly(A) signal/note="human growth hormone polyadenylation signal"promoter 3307..3604/label=SV40 promoter/note="SV40 enhancer and early promoter"CDS 3658..4452/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 5104..5238/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(5678..6266)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(6437..7297)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(7298..7402)/gene="bla"/label=AmpR promoterrep_origin 7429..7884/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"ORIGIN1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcaatgaac1021 gaggaggcca tctgcagcgc cctgcccacc atcccgtacc gaaagctcgc tgacctgtgc1081 tacttgagcc gtggcgcgtc cggcaccgtg tcgtccgccc gccacgcaga ctggcgcatc1141 caggtggccg taaagcactt gcacatccac accccactgc ttgacagtga aagaaatgat1201 gtcttaagag aagctgaaat tttacacaaa gctagattta gttacattct tccaattttg1261 ggaatttgca atgagcctga atttttggga atagttaccg aatatatgcc aaatggatcg1321 ttaaatgaac tcctacatag gaaaaatgaa tatcctgatg ttccttggcc tttgagattt1381 cgtattttgc atgaaattgc actaggtgta aattacctgc acaatatgaa tcctccttta1441 cttcaccatg atctgaagac tcaaaatatc ttattggata atgaatttca tgttaagatt1501 gcagattttg gtttatcaaa atggcgtatg atgtccctct cacaatcacg aagtattaaa1561 tctgctccag aaggagggac aattatctat atgccacctg aaaactatga acctggacaa1621 aaatcaaggg ctactgtcaa gcatgatata tatagttatg cagttatcac atgggaagtc1681 ttatccagaa aacagccttt tgaagaagtc accaatcctt tgcagattat gtatagcgtg1741 tcacaaggac atcgacctga cactagcgaa gaaagtttgc cgtttgatat acctcatcga1801 gcactgatga tctctctaat agaaagtgga tgggcacaaa atccagatga aagaccatct1861 ttcttaaaat gtttaataga acttgaacct gttctgagaa tatttgaaga tataactttc1921 cttgaagctg ttattcaact aaagaaaaca aagttgcaaa gtgcttcgag tactgttcac1981 ttatgtgaca agaagaaaac agagttatat ccgaacacat ctgtaaatca tggttcacaa2041 gaggaatcat gtggatcttc gcagcttcat aaacctagta attctccagg aacctcaaag2101 tccctgtcag ctactcaaga cagtgacttt ttatctagaa aaactcaaga cttttccacg2161 ccacgtcagt gtccagtaaa tcgcagttgg gacagtaaca tttctgtggc tcaaagggca2221 acattctgtg atcacaagac caccccatgc tctttagcaa taataaatcc actctcagcc2281 gaaggaaatt cagagcgatt tcagcctggt atagcccaac agtggatcca gagtaaaagg2341 gaagacattg tgaaccagat gacggaagcc tgtcttaacc aatctctgga tgccctcctg2401 tccagggatt tgatcatgaa ggaggactat gaactcatta gtaccaaacc tacaagaacc2461 tcaaaagtca gacaattact ggataccact gacatccaag gagaagaatt tgccagagtt2521 atagtacaaa agttgaaaga taacaagcaa atgggactcc agccttaccc agaaatactt2581 gtggtttctc gaccaccatc tttaaattta ttttagggta ccagtcgact ctagaggatc2641 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc2701 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt2761 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga2821 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt2881 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt2941 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac3001 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac3061 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc3121 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc3181 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt3241 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc3301 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc3361 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc3421 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc3481 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt3541 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt3601 ttggaggaat tgatcagctt gggatctgat caagagacag gatgaggatc gtttcgcatg3661 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc3721 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg3781 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag3841 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc3901 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat3961 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg4021 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc4081 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag4141 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc4201 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc4261 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata服务时间:24小时在线客服
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