• 规格：20μl质粒
• 启动子：CMV promoter
• 复制子：Ori，F1 ori
• 终止子：hGH poly(A) signal
• 质粒分类：基因文库质粒；其它基因质粒；猪源基因质粒
• 质粒大小：7884bp
• 质粒标签：N-3×FLAG
• 原核抗性：氨苄青霉素Amp（100μg/ml）
• 筛选标记：新霉素Neo/G418
• 克隆菌株：DH5α等大肠杆菌
• 表达宿主：293T等哺乳细胞
• 质粒简介：            p3×FLAG-CMV-10-RIP2-p质粒是一个猪源基因过表达质粒，CMV启动子驱动3×FLAG标签和RIP2基因融合表达。p3×FLAG-CMV-10-RIP2-p质粒由淼灵生物开发。
             PREDICTED: Sus scrofa receptor interacting serine/threonine kinase 2 (RIPK2), transcript variant X1, mRNA.NCBI Reference Sequence: XM_021089139.1.
• 质粒图谱1： png/1-1FQ6154102R6.png

质粒序列： 点击查看序列

LOCUS       Exported                7884 bp ds-DNA     circular SYN 16-AUG-2017

DEFINITION  synthetic circular DNA

KEYWORDS    p3×FLAG-CMV-10-RIP2-p.dna

FEATURES             Location/Qualifiers

     source          1..7884

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        318..697

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        698..901

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             931..996

                     /codon_start=1

                     /product="three tandem FLAG(R) epitope tags, followed by an

                     enterokinase cleavage site"

                     /label=3xFLAG

                     /translation="DYKDHDGDYKDHDIDYKDDDDK"

     CDS             1015..2616

                     /codon_start=1

                     /label=RIP2-p

                     /translation="MNEEAICSALPTIPYRKLADLCYLSRGASGTVSSARHADWRIQVA

                     VKHLHIHTPLLDSERNDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLN

                     ELLHRKNEYPDVPWPLRFRILHEIALGVNYLHNMNPPLLHHDLKTQNILLDNEFHVKIA

                     DFGLSKWRMMSLSQSRSIKSAPEGGTIIYMPPENYEPGQKSRATVKHDIYSYAVITWEV

                     LSRKQPFEEVTNPLQIMYSVSQGHRPDTSEESLPFDIPHRALMISLIESGWAQNPDERP

                     SFLKCLIELEPVLRIFEDITFLEAVIQLKKTKLQSASSTVHLCDKKKTELYPNTSVNHG

                     SQEESCGSSQLHKPSNSPGTSKSLSATQDSDFLSRKTQDFSTPRQCPVNRSWDSNISVA

                     QRATFCDHKTTPCSLAIINPLSAEGNSERFQPGIAQQWIQSKREDIVNQMTEACLNQSL

                     DALLSRDLIMKEDYELISTKPTRTSKVRQLLDTTDIQGEEFARVIVQKLKDNKQMGLQP

                     YPEILVVSRPPSLNLF"

     polyA_signal    2643..3265

                     /label=hGH poly(A) signal

                     /note="human growth hormone polyadenylation signal"

     promoter        3307..3604

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     CDS             3658..4452

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5104..5238

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(5678..6266)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6437..7297)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7298..7402)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      7429..7884

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

ORIGIN

        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct

       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg

      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa

      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta

      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc

      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg

      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc

      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat

      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc

      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga

      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg

      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac

      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt

      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc

      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc

      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa

      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcaatgaac

     1021 gaggaggcca tctgcagcgc cctgcccacc atcccgtacc gaaagctcgc tgacctgtgc

     1081 tacttgagcc gtggcgcgtc cggcaccgtg tcgtccgccc gccacgcaga ctggcgcatc

     1141 caggtggccg taaagcactt gcacatccac accccactgc ttgacagtga aagaaatgat

     1201 gtcttaagag aagctgaaat tttacacaaa gctagattta gttacattct tccaattttg

     1261 ggaatttgca atgagcctga atttttggga atagttaccg aatatatgcc aaatggatcg

     1321 ttaaatgaac tcctacatag gaaaaatgaa tatcctgatg ttccttggcc tttgagattt

     1381 cgtattttgc atgaaattgc actaggtgta aattacctgc acaatatgaa tcctccttta

     1441 cttcaccatg atctgaagac tcaaaatatc ttattggata atgaatttca tgttaagatt

     1501 gcagattttg gtttatcaaa atggcgtatg atgtccctct cacaatcacg aagtattaaa

     1561 tctgctccag aaggagggac aattatctat atgccacctg aaaactatga acctggacaa

     1621 aaatcaaggg ctactgtcaa gcatgatata tatagttatg cagttatcac atgggaagtc

     1681 ttatccagaa aacagccttt tgaagaagtc accaatcctt tgcagattat gtatagcgtg

     1741 tcacaaggac atcgacctga cactagcgaa gaaagtttgc cgtttgatat acctcatcga

     1801 gcactgatga tctctctaat agaaagtgga tgggcacaaa atccagatga aagaccatct

     1861 ttcttaaaat gtttaataga acttgaacct gttctgagaa tatttgaaga tataactttc

     1921 cttgaagctg ttattcaact aaagaaaaca aagttgcaaa gtgcttcgag tactgttcac

     1981 ttatgtgaca agaagaaaac agagttatat ccgaacacat ctgtaaatca tggttcacaa

     2041 gaggaatcat gtggatcttc gcagcttcat aaacctagta attctccagg aacctcaaag

     2101 tccctgtcag ctactcaaga cagtgacttt ttatctagaa aaactcaaga cttttccacg

     2161 ccacgtcagt gtccagtaaa tcgcagttgg gacagtaaca tttctgtggc tcaaagggca

     2221 acattctgtg atcacaagac caccccatgc tctttagcaa taataaatcc actctcagcc

     2281 gaaggaaatt cagagcgatt tcagcctggt atagcccaac agtggatcca gagtaaaagg

     2341 gaagacattg tgaaccagat gacggaagcc tgtcttaacc aatctctgga tgccctcctg

     2401 tccagggatt tgatcatgaa ggaggactat gaactcatta gtaccaaacc tacaagaacc

     2461 tcaaaagtca gacaattact ggataccact gacatccaag gagaagaatt tgccagagtt

     2521 atagtacaaa agttgaaaga taacaagcaa atgggactcc agccttaccc agaaatactt

     2581 gtggtttctc gaccaccatc tttaaattta ttttagggta ccagtcgact ctagaggatc

     2641 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc

     2701 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt

     2761 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga

     2821 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt

     2881 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt

     2941 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac

     3001 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac

     3061 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc

     3121 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc

     3181 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt

     3241 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc

     3301 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc

     3361 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc

     3421 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc

     3481 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt

     3541 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt

     3601 ttggaggaat tgatcagctt gggatctgat caagagacag gatgaggatc gtttcgcatg

     3661 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc

     3721 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg

     3781 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag

     3841 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc

     3901 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat

     3961 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg

     4021 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc

     4081 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag

     4141 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc

     4201 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc

     4261 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata