- 商品货号:FH1158
- 规格:10μl质粒
- 启动子:Lac promoter,T3 promoter
- 复制子:p15A ori
- 质粒分类:基因文库质粒;其它基因质粒;其它种源质粒
- 质粒大小:5198bp
- 原核抗性:卡那霉素Kan(50μg/ml)
- 克隆菌株:DH5α等大肠杆菌
- 备注:低拷贝
- 质粒简介: pInt质粒提供提供链霉菌噬菌体phiC31基因,其参考文献:A phage integrase directs efficient site-specific integration in human cells.
- 质粒图谱1: png/1-1FQ51K30aN.png
质粒序列: 点击查看序列LOCUS Exported 5198 bp ds-DNA circular SYN 15-AUG-2017KEYWORDS pInt.dnaFEATURES Location/Qualifierssource 1..5198/organism="synthetic DNA construct"/mol_type="other DNA"CDS complement(16..1833)/codon_start=1/product="integrase from phage phi-C31"/label=phage phi-C31 integrase/translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGGRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPIVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELGGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREIKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILRDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAWLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERANLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEAPKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIEKRASITWAKPPTDDDEDDAQDGTEDVAA"promoter complement(1897..1915)/label=T3 promoter/note="promoter for bacteriophage T3 RNA polymerase"primer_bind complement(1936..1952)/label=M13 rev/note="common sequencing primer, one of multiple similarvariants"protein_bind 1960..1976/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."promoter complement(1984..2014)/label=lac promoter/note="promoter for the E. coli lac operon"protein_bind 2029..2050/label=CAP binding site/bound_moiety="E. coli catabolite activator protein"/note="CAP binding activates transcription in the presenceof cAMP."rep_origin 2662..3206/direction=RIGHT/label=p15A ori/note="Plasmids containing the medium-copy-number p15Aorigin of replication can be propagated in E. coli cellsthat contain a second plasmid with the ColE1 origin."CDS 3801..4616/codon_start=1/gene="aph(3')-Ia"/product="aminoglycoside phosphotransferase"/label=KanR/note="confers resistance to kanamycin in bacteria or G418(Geneticin(R)) in eukaryotes"/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"ORIGIN1 gatccgggtg tctcgctacg ccgctacgtc ttccgtgccg tcctgggcgt cgtcttcgtc61 gtcgtcggtc ggcggcttcg cccacgtgat cgaagcgcgc ttctcgatgg gcgttccctg121 ccccctgccc gtagtcgact tcgtgacaac gatcttgtct acgaagagcc cgacgaacac181 gcgcttgtcg tctactgacg cgcgccccca ccacgactta gggccggtcg ggtcagcgtc241 ggcgtcttcg gggaaccatt ggtcaagggg aagcttcggg gcttcggcgg cttcaagttc301 ggcaagccgc tcttccgccc cttgctgccg gagcgtcagc gctgcctgtt gcttccggaa361 gtgcttcctg ccaacgggtc cgtcgtacgc gcctgccgcg cggtcttcgt acagctcttc421 aagggcgttc agggcgtcgg cgcgctccgc aacaaggttc gcccgttcgc cgctcttctc481 aggcgcctca gtgagcttgc cgaagcgtcg ggcggcttcc cacagaagcg ccaacgtctc541 ttcgtcgcct tcggcgtgcc tgatcttgtt gaagatgcgt tccgcaacga acttgtcgag601 tgccgccatg ctgacgttgc acgtgccttc gtgctgccca ggtgcggacg ggtcgaccac661 cttccggcga cggcagcggt aagagtcctt gatcgattct tccccgcgct tcgaagtcat721 gacggcgcca cactcgcagt acagcttgtc catggcggac agaatggctt gcccccggga781 aagccccttg ccgcgccccc tgccgtccaa ccacgcctga agctcatacc actcagcggg841 ctcgatgatc ggtccgcaat caagctcgac cggccggagc gtgatcgggt cgcgctgaat901 gcggtaaccc tcaatcttcg tggtcggcgt gccgtccggc ttcttcttgt agatcacctc961 agcggcgaag cccgcaatac gcgggtcccg aaggattcgc ataacggttg ccgggtccca1021 ggcgcttgaa gcggtcttct tcccaatcgt ctcgccccgg gtcggcacgg cgtcagcgtc1081 catgcgctta caaagccccg tgatgctgcc cgggtgaatg gcggcttgac tgcccggctt1141 gaagggaagg tgtttgtgcg tcttgatctc acgccaccac caccggatta cgtcgggctc1201 gaactcgaag ggtccggtaa ggggagtggt cgagtgcgca agcttgttga tgacgacatt1261 gaccattcgg ccgttgcgcg tgatctcctt cgtctccgaa acaagctcga agccgtaagg1321 cgccttcccg ccgacgtacc cgcccaattc gcgctgaagg ttcttcgtgt cgagaatctt1381 cgccgacttc agcgaagatt ctttgtgcga cgcgtcgagc cgcataatca ggtgaatcag1441 gtccatgacg tttccctgcc ggaagacgcc ttcctgagtg gaaacaatcg tcacgcccag1501 ggcgagcaat tccgagacaa tcggaatcgc gtccatgacc ttcaggcgcg agaagcgcga1561 cacgtcatag acaatgatca tgttgagccg cccggcgcgg cattcgttca ggatgcgttc1621 gaactccggg cgctccgccg tcccgaacgc cgacgtgccc ggcgcttcgc tgaaatgccc1681 gacgaacctg aaccggcccc cgtcgcgctc gacttcgcgc tgaaggtcgg ccgccttgtc1741 ttcgttggcg ctacgctgtg tcgctgggct tgctgcgctc gaattctcgc gctcgcgcga1801 ctgacggtcg taagcacccg cgtacgtgtc caccccggtc acaacccctt gtgtcatgtc1861 ggcgacccta cgactagtga gctccagctt ttgttccctt tagtgagggt taattgcgcg1921 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc1981 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta2041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca2101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc2161 cgctcggcca aaaaggcctg cagcaatggc aacaacgttg cgcaaactat taactggcga2221 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc2281 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc2341 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg2401 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat2461 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata2521 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct2581 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga2641 ccccttaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga2701 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt2761 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc2821 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca2881 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc2941 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc3001 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg3061 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt3121 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct3181 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt3241 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg3301 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta3361 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca3421 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc3481 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc3541 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt3601 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct3661 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat3721 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa3781 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc3841 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg3901 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc3961 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact4021 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg4081 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc4141 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga4201 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat4261 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc4321 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg4381 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt4441 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga4501 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg4561 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag4621 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct4681 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa4741 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa4801 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg4861 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctcaaaga tgcaggggta4921 aaagctaacc gcatctttac cgacaaggca tccggcagtt caacagatcg ggaagggctg4981 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggtgaagaa gctcgaccgt5041 cttggccgcg acaccgccga catgatccaa ctgataaaag agtttgatgc tcagggtgta5101 gcggttcggt ttattgacga cgggatcagt accgacggtg atatggggca aatggtggtc5161 accatcctgt cggctgtggc acaggctgaa cgccggag//
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