- 商品货号:FH1157
- 规格:10μl质粒
- 启动子:T7/lac promoter
- 复制子:ori,F1 ori
- 终止子:T7 terminator
- 质粒分类:基因文库质粒;其它基因质粒;其它种源质粒
- 质粒大小:7297bp
- 质粒标签:C-6×His
- 原核抗性:卡那霉素Kan(50μg/ml)
- 克隆菌株:DH5α等大肠杆菌
- 表达宿主:BL21(DE3)大肠杆菌
- 诱导方式:IPTG或乳糖及其类似物
- 质粒简介: pET28a-PPK质粒可以转化到含DE3的大肠杆菌中,通过IPTG的诱导表达PPK基因。
- 质粒图谱1: png/1-1FQ5153622148.png
质粒序列: 点击查看序列LOCUS Exported 7297 bp ds-DNA circular SYN 15-AUG-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..7297/organism="synthetic DNA construct"/mol_type="other DNA"terminator 26..73/label=T7 terminator/note="transcription terminator for bacteriophage T7 RNApolymerase"CDS complement(140..157)/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"misc_feature complement(164..2227)/label=PPKRBS 2234..2256/note="efficient ribosome binding site from bacteriophageT7 gene 10 (Olins and Rangwala, 1989)"protein_bind 2271..2295/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."promoter complement(2296..2314)/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"promoter 2623..2700/gene="lacI"/label=lacI promoterCDS 2701..3783/codon_start=1/gene="lacI"/product="lac repressor"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"CDS 4592..4783/codon_start=1/gene="rop"/product="Rop protein, which maintains plasmids at low copynumber"/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL"misc_feature 4885..5027/label=bom/note="basis of mobility region from pBR322"rep_origin complement(5213..5801)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS 5923..6738/codon_start=1/gene="aph(3')-Ia"/product="aminoglycoside phosphotransferase"/label=KanR/note="confers resistance to kanamycin in bacteria or G418(Geneticin(R)) in eukaryotes"/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"rep_origin complement(6831..7286)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"ORIGIN1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttcaggt tgttcaagag181 atttgatgta atcgtagata gcaagttgag cacgaacttt acgacggttg ccacgaggaa241 cgtaacggtt agaaagttct ttatcgatgt aacgagcttt aactgtatca gagaaaagga301 tatcgatgat atcaagaaca cgttgtttaa gacgaggatc aagaagaggt gtagcaactt361 cgatacggta atcgatgtta cgtgtcatcc aatcagcaga agaaaggtaa acttttttat421 cgccgccgtt ttcgaagatg taaacacgat catgttcaag gtaacgatca acgatagaga481 tagcacggat gttatcagag atgccttcaa ggttagggat aagagagcac atgccacgaa541 caagaaggtt aacaggaacg ccagaagaag aagcagcgta aagacgatca acaaggcctt601 tatcaacaag gttgttaagt ttaagtgtga tgccagaagg aaggccttgt tgagcgttag661 cgatttcacg atcaaccatt tcgtaaagaa gacgacgaga gttttgagga gaaaccataa721 ggtaatcgaa tgtaacagga cggtaagggt tttcgatgaa gttgaaaaca cgacgaactt781 cgtttgtgat acgagcatca gctgtaagaa gagagtaatc tgtgtaaaga cgagctgttt841 tttcgttgaa gttgcctgtg ccgatatgag cgtaacgaac aacttcgccg ttttctttac901 gagagataag gaaaagttta gcatggattt taaggccagg agcagagaag ataacatgaa961 cgccagcttc tgtaagacgt ttagcccaat ggatgttagc ttcttcatcg aaacgagctt1021 gaagttcaac aacaactgta acttttttgc cgttatgagc agcatggatc atagaatcga1081 tgatacgaga atctttagca acacggtaga tgttgatttt gatagcaaga acagaaggat1141 cgaaagaagc ttgacgaaga agttcaagaa catgttcgaa tgtatggtaa gggtagtaaa1201 gaagaacatc acgttcacgg atagcatcga agccgttacg gaattgagct ttatcgaacc1261 agatatgacg aagacgagga agaggtttgt taacaaggtt agctttgcca acgttaggga1321 agttgatgaa atctttgaag ttatggtaac ggccgccagg aacgatagaa tcgtaacgag1381 agattgtaag tttttcacga agaacttcaa caagagcgtt aggcatatca cgttggtaaa1441 cgaaacgaac aggttcagct gtaagacgtt gtttaagaga agaagacata agttccataa1501 gagaagcttc catttcatga acaagatcgt attcagcatc acgtgtcatt ttcatagagt1561 aagcgttaag agcatcgtaa tcgaagaagc ctttgaagat atcatcaagg cagtaacgaa1621 ggatgttatc aagaaggatc ataggtttac gacgacgagg agcttcagga ggaaggttaa1681 cgaaacgagg aactttatca gaagggattt caagaagagc gtaacggatt gtatcgccac1741 ggatgatttc aacagcaagg tatgtgtaat catctttaag gaattgaaca agatctgtat1801 cagggttgat aaggataggt gtgatatgtt gacgaaggta ttgtttgaag taatgacgaa1861 gccagttttg ttggttaaca gaaagttgac gttcgttgat aaggaagatt tggttacgag1921 ccatttcaag aagaagttcg ttgtaaaggc catcgaattc ttgatcagct ttaagaacac1981 gagattggat tttgccaaga agatgacgag aatgagagtt agagccttgt tcttcagaga2041 tgatgatacg acgtttaagt tcagcgaaac gaactttgta gaattcatca aggttgttag2101 agtagatgcc aaggaaacgc atacgttcga taagagggtt agatttatca gcagcttctt2161 gaagaacacg ttcgttgaaa gaaagccaag aaagttcttt ttcgatgtaa agtttttctt2221 ggcccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta2281 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc2341 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct2401 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt2461 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct2521 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct2581 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc2641 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat2701 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt2761 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg2821 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag2881 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc2941 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa3001 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt3061 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc3121 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt3181 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag3241 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc3301 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg3361 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact3421 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc3481 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca3541 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc3601 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc3661 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc3721 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag3781 tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat3841 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt3901 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct3961 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct4021 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa4081 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat4141 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa4201 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg4261 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc服务时间:24小时在线客服
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