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- 规格：20μl质粒
- 质粒图谱1： png/1-1F930154949225.png
质粒序列： 点击查看序列LOCUS Exported 4474 bp ds-DNA circular SYN 30-SEP-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS .SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 4474)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..4474/organism="synthetic DNA construct"/mol_type="other DNA"misc_feature 63..95/label=myristolation signalmisc_feature 96..1535/label=akt-T308A/S473ACDS 1536..1562/codon_start=1/product="HA (human influenza hemagglutinin) epitope tag"/label=HA/translation="YPYDVPDYA"promoter complement(1632..1650)/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"primer_bind complement(1657..1673)/label=M13 fwd/note="common sequencing primer, one of multiple similarvariants"rep_origin complement(1814..2269)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 2296..2400/gene="bla"/label=AmpR promoterCDS 2401..3261/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin 3432..4020/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"primer_bind 4406..4422/label=M13 rev/note="common sequencing primer, one of multiple similarvariants"promoter 4443..4461/label=T3 promoter/note="promoter for bacteriophage T3 RNA polymerase"ORIGIN1 agctggccat tacggccgcg gccgcctcta gaggatctgg taccacgcgt atcgataagc61 ttatggggag cagcaagagc aagcccaagt ctagaatgaa cgacgtagcc attgtgaagg121 agggctggct gcacaaacga ggggaatata ttaaaacctg gcggccacgc tacttcctcc181 tcaagaacga tggcaccttt attggctaca aggaacggcc tcaggatgtg gatcagcgag241 agtccccact caacaacttc tcagtggcac aatgccagct gatgaagaca gagcggccaa301 ggcccaacac ctttatcatc cgctgcctgc agtggaccac agtcattgag cgcaccttcc361 atgtggaaac gcctgaggag cgggaagaat gggccaccgc cattcagact gtggccgatg421 gactcaagag gcaggaagaa gagacgatgg acttccgatc aggctcaccc agtgacaact481 caggggctga agagatggag gtgtccctgg ccaagcccaa gcaccgtgtg accatgaacg541 agtttgagta cctgaaacta ctgggcaagg gcacctttgg gaaagtgatt ctggtgaaag601 agaaggccac aggccgctac tatgccatga agatcctcaa gaaggaggtc atcgtcgcca661 aggatgaggt tgcccacacg cttactgaga accgtgtcct gcagaactct aggcatccct721 tccttacggc cctcaagtac tcattccaga cccacgaccg cctctgcttt gtcatggagt781 atgccaacgg gggcgagctc ttcttccacc tgtctcgaga gcgcgtgttc tccgaggacc841 gggcccgctt ctatggtgcg gagattgtgt ctgccctgga ctacttgcac tccgagaaga901 acgtggtgta ccgggacctg aagctggaga acctcatgct ggacaaggac gggcacatca961 agataacgga cttcgggctg tgcaaggagg ggatcaagga tggtgccact atgaaggcat1021 tctgcggaac gccggagtac ctggcccctg aggtgctgga ggacaacgac tacggccgtg1081 cagtggactg gtgggggctg ggcgtggtca tgtatgagat gatgtgtggc cgcctgccct1141 tctacaacca ggaccacgag aagctgttcg agctgatcct catggaggag atccgcttcc1201 cgcgcacact cggccctgag gccaagtccc tgctctccgg gctgctcaag aaggacccta1261 cacagaggct cggtgggggc tctgaggatg ccaaggagat catgcagcac cggttctttg1321 ccaacatcgt gtggcaggat gtgtatgaga agaagctgag cccacctttc aagccccagg1381 tcacctctga gactgacacc aggtatttcg atgaggagtt cacagctcag atgatcacca1441 tcacgccgcc tgatcaagat gacagcatgg agtgtgtgga cagtgagcgg aggccgcact1501 tcccccagtt cgcctactca gccagtggca cagcctaccc atacgatgtt ccagattacg1561 cttctagatg aattcacgcg taagcttatc gatgtcgacg ggcccggccg cctcggccgt1621 acccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg1681 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc1741 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct1801 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt1861 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa1921 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag1981 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt2041 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac2101 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag2161 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg2221 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact2281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg2341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt2401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct2461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca2521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc2581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc2641 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg2701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta2761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc2821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt2881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg2941 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct3001 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc3061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct3121 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac3181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc3241 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat3301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg3361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc3421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa3481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag3541 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta3601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta3661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag3721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg3781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg3841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag3901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc3961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa4021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg4081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct4141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa4201 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg4261 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag4321 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga4381 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc4441 gaaattaacc ctcactaaag ggaacaaaag ctgg//
