• 商品货号：FH1820

• 规格：10μl质粒
• 原核抗性：氨苄青霉素
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：                pCDNA3.1-H1N1-Polymerase 是一个甲型流感病毒聚合酶基因哺乳表达质粒。Influenza A virus (A/Puerto Rico/8/34(H1N1)) polymerase 1 gene, complete cds；GenBank: J02151.1.
• 质粒图谱1： png/1-1P11411360D38.png

质粒序列： 点击查看序列

LOCUS       Exported                7685 bp ds-DNA     circular SYN 14-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..7685

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        202..581

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        582..785

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        830..848

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     regulatory      890..899

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     misc_feature    896..3166

                     /label=H1N1-polymerase 1

     CDS             3182..3205

                     /codon_start=1

                     /product="8xHis affinity tag"

                     /label=8xHis

                     /translation="HHHHHHHH"

     polyA_signal    3252..3476

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      3522..3950

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3964..4293

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4144..4279

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4360..5154

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5328..5449

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(5900..6485)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6656..7516)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7517..7621)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt

       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct

      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg

      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta

      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg

      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc

      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa

      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca

      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta

      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt

      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg

      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca

      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca

      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc

      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgga

      901 tgtcaatccg accttacttt tcttaaaagt gccagcacaa aatgctataa gcacaacttt

      961 cccttatacc ggagaccctc cttacagcca tgggacagga acaggataca ccatggatac

     1021 tgtcaacagg acacatcagt actcagaaaa ggcaagatgg acaacaaaca ccgaaactgg

     1081 agcaccgcaa ctcaacccga ttgatgggcc actgccagaa gacaatgaac caagtggtta

     1141 tgcccaaaca gattgtgtat tggaagcaat ggctttcctt gaggaatccc atcctggtat

     1201 ttttgaaaac tcgtgtattg aaacgatgga ggttgttcag caaacacgag tagacaagct

     1261 gacacaaggc cgacagacct atgactggac tttaaataga aaccagcctg ctgcaacagc

     1321 attggccaac acaatagaag tgttcagatc aaatggcctc acggccaatg agtctggaag

     1381 gctcatagac ttccttaagg atgtaatgga gtcaatgaaa aaagaagaaa tggggatcac

     1441 aactcatttt cagagaaaga gacgggtgag agacaatatg actaagaaaa tgataacaca

     1501 gagaacaata ggtaaaagga aacagagatt gaacaaaagg agttatctaa ttagagcatt

     1561 gaccctgaac acaatgacca aagatgctga gagagggaag ctaaaacgga gagcaattgc

     1621 aaccccaggg atgcaaataa gggggtttgt atactttgtt gagacactgg caaggagtat

     1681 atgtgagaaa cttgaacaat cagggttgcc agttggaggc aatgagaaga aagcaaagtt

     1741 ggcaaatgtt gtaaggaaga tgatgaccaa ttctcaggac accgaacttt ctttgaccat

     1801 cactggagat aacaccaaat ggaacgaaaa tcagaatcct cggatgtttt tggccatgat

     1861 cacatatatg accagaaatc agcccgaatg gttcagaaat gttctaagta ttgctccaat

     1921 aatgttctca aacaaaatgg cgagactggg aaaagggtat atgtttgaga gcaagagtat

     1981 gaaacttaga actcaaatac ctgcagaaat gctagcaagc attgatttga aatatttcaa

     2041 tgattcaaca agaaagaaga ttgaaaaaat ccgaccgctc ttaatagagg ggactgcatc

     2101 attgagccct ggaatgatga tgggcatgtt caatatgtta agcactgtat taggcgtctc

     2161 catcctgaat cttggacaaa agagatacac caagactact tactggtggg atggtcttca

     2221 atcctctgac gattttgctc tgattgtgaa tgcacccaat catgaaggga ttcaagccgg

     2281 agtcgacagg ttttatcgaa cctgtaagct acatggaatc aatatgagca agaaaaagtc

     2341 ttacataaac agaacaggta catttgaatt cacaagtttt ttctatcgtt atgggtttgt

     2401 tgccaatttc agcatggagc ttcccagttt tggtgtgtct gggagcaacg agtcagcgga

     2461 catgagtatt ggagttactg tcatcaaaaa caatatgata aacaatgatc ttggtccagc

     2521 aacagctcaa atggcccttc agttgttcat caaagattac aggtacacgt accgatgcca

     2581 tagaggtgac acacaaatac aaacccgaag atcatttgaa ataaagaaac tgtgggagca

     2641 aacccgttcc aaagctggac tgctggtctc cgacggaggc ccaaatttat acaacattag

     2701 aaatctccac attcctgaag tctgcctaaa atgggaattg atggatgagg attaccaggg

     2761 gcgtttatgc aacccactga acccatttgt cagccataaa gaaattgaat caatgaacaa

     2821 tgcagtgatg atgccagcac atggtccagc caaaaacatg gagtatgatg ctgttgcaac

     2881 aacacactcc tggatcccca aaagaaatcg atccatcttg aatacaagtc aaagaggagt

     2941 acttgaagat gaacaaatgt accaaaggtg ctgcaattta tttgaaaaat tcttccccag

     3001 cagttcatac agaagaccag tcgggatatc cagtatggtg gaggctatgg tttccagagc

     3061 ccgaattgat gcacggattg atttcgaatc tggaaggata aagaaagaag agttcactga

     3121 gatcatgaag atctgttcca ccattgaaga gctcagacgg caaaaaggag gcggcggttc

     3181 ccatcaccac catcaccacc atcattgact cgagtctaga gggcccgttt aaacccgctg

     3241 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc

     3301 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc

     3361 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa

     3421 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc

     3481 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc

     3541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct

     3601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg

     3661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga

     3721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt

     3781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg

     3841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc

     3901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg

     3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa

     4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc

     4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg

     4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt

     4201 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga

     4261 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt

     4321 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg

     4381 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag

     4441 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt

     4501 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta

     4561 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg