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- 规格：20μl质粒
- 原核抗性：氨苄青霉素
- 筛选标记：遗传霉素G418
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pCDNA3.1-H1N1-Polymerase 是一个甲型流感病毒聚合酶基因哺乳表达质粒。Influenza A virus (A/Puerto Rico/8/34(H1N1)) polymerase 1 gene, complete cds；GenBank: J02151.1.
- 质粒图谱1： png/1-1P11411360D38.png
质粒序列： 点击查看序列LOCUS Exported 7685 bp ds-DNA circular SYN 14-JAN-2018DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..7685/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 202..581/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 582..785/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 830..848/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"regulatory 890..899/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"misc_feature 896..3166/label=H1N1-polymerase 1CDS 3182..3205/codon_start=1/product="8xHis affinity tag"/label=8xHis/translation="HHHHHHHH"polyA_signal 3252..3476/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 3522..3950/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 3964..4293/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 4144..4279/label=SV40 ori/note="SV40 origin of replication"CDS 4360..5154/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 5328..5449/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(5900..6485)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(6656..7516)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(7517..7621)/gene="bla"/label=AmpR promoterORIGIN1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgga901 tgtcaatccg accttacttt tcttaaaagt gccagcacaa aatgctataa gcacaacttt961 cccttatacc ggagaccctc cttacagcca tgggacagga acaggataca ccatggatac1021 tgtcaacagg acacatcagt actcagaaaa ggcaagatgg acaacaaaca ccgaaactgg1081 agcaccgcaa ctcaacccga ttgatgggcc actgccagaa gacaatgaac caagtggtta1141 tgcccaaaca gattgtgtat tggaagcaat ggctttcctt gaggaatccc atcctggtat1201 ttttgaaaac tcgtgtattg aaacgatgga ggttgttcag caaacacgag tagacaagct1261 gacacaaggc cgacagacct atgactggac tttaaataga aaccagcctg ctgcaacagc1321 attggccaac acaatagaag tgttcagatc aaatggcctc acggccaatg agtctggaag1381 gctcatagac ttccttaagg atgtaatgga gtcaatgaaa aaagaagaaa tggggatcac1441 aactcatttt cagagaaaga gacgggtgag agacaatatg actaagaaaa tgataacaca1501 gagaacaata ggtaaaagga aacagagatt gaacaaaagg agttatctaa ttagagcatt1561 gaccctgaac acaatgacca aagatgctga gagagggaag ctaaaacgga gagcaattgc1621 aaccccaggg atgcaaataa gggggtttgt atactttgtt gagacactgg caaggagtat1681 atgtgagaaa cttgaacaat cagggttgcc agttggaggc aatgagaaga aagcaaagtt1741 ggcaaatgtt gtaaggaaga tgatgaccaa ttctcaggac accgaacttt ctttgaccat1801 cactggagat aacaccaaat ggaacgaaaa tcagaatcct cggatgtttt tggccatgat1861 cacatatatg accagaaatc agcccgaatg gttcagaaat gttctaagta ttgctccaat1921 aatgttctca aacaaaatgg cgagactggg aaaagggtat atgtttgaga gcaagagtat1981 gaaacttaga actcaaatac ctgcagaaat gctagcaagc attgatttga aatatttcaa2041 tgattcaaca agaaagaaga ttgaaaaaat ccgaccgctc ttaatagagg ggactgcatc2101 attgagccct ggaatgatga tgggcatgtt caatatgtta agcactgtat taggcgtctc2161 catcctgaat cttggacaaa agagatacac caagactact tactggtggg atggtcttca2221 atcctctgac gattttgctc tgattgtgaa tgcacccaat catgaaggga ttcaagccgg2281 agtcgacagg ttttatcgaa cctgtaagct acatggaatc aatatgagca agaaaaagtc2341 ttacataaac agaacaggta catttgaatt cacaagtttt ttctatcgtt atgggtttgt2401 tgccaatttc agcatggagc ttcccagttt tggtgtgtct gggagcaacg agtcagcgga2461 catgagtatt ggagttactg tcatcaaaaa caatatgata aacaatgatc ttggtccagc2521 aacagctcaa atggcccttc agttgttcat caaagattac aggtacacgt accgatgcca2581 tagaggtgac acacaaatac aaacccgaag atcatttgaa ataaagaaac tgtgggagca2641 aacccgttcc aaagctggac tgctggtctc cgacggaggc ccaaatttat acaacattag2701 aaatctccac attcctgaag tctgcctaaa atgggaattg atggatgagg attaccaggg2761 gcgtttatgc aacccactga acccatttgt cagccataaa gaaattgaat caatgaacaa2821 tgcagtgatg atgccagcac atggtccagc caaaaacatg gagtatgatg ctgttgcaac2881 aacacactcc tggatcccca aaagaaatcg atccatcttg aatacaagtc aaagaggagt2941 acttgaagat gaacaaatgt accaaaggtg ctgcaattta tttgaaaaat tcttccccag3001 cagttcatac agaagaccag tcgggatatc cagtatggtg gaggctatgg tttccagagc3061 ccgaattgat gcacggattg atttcgaatc tggaaggata aagaaagaag agttcactga3121 gatcatgaag atctgttcca ccattgaaga gctcagacgg caaaaaggag gcggcggttc3181 ccatcaccac catcaccacc atcattgact cgagtctaga gggcccgttt aaacccgctg3241 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc3301 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc3361 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa3421 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc3481 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc3541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct3601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg3661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga3721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt3781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg3841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc3901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt4201 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga4261 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt4321 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg4381 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag4441 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt4501 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta4561 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
