• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：          pCDNA3.1-H1N1-ORF1是一个甲型流感病毒基因哺乳细胞表达质粒。Influenza A virus (A/Puerto Rico/8/1934(H1N1)) ORF1, genomic RNA；GenBank: V01106.1
• 质粒图谱1： png/1-1P114112934605.png

质粒序列： 点击查看序列

LOCUS       Exported                7562 bp ds-DNA     circular SYN 14-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..7562

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        202..581

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        582..785

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        830..848

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     regulatory      890..899

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     misc_feature    896..3043

                     /label=PDB

     CDS             3059..3082

                     /codon_start=1

                     /product="8xHis affinity tag"

                     /label=8xHis

                     /translation="HHHHHHHH"

     polyA_signal    3129..3353

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      3399..3827

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3841..4170

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4021..4156

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4237..5031

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5205..5326

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(5777..6362)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6533..7393)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7394..7498)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt

       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct

      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg

      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta

      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg

      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc

      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa

      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca

      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta

      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt

      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg

      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca

      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca

      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc

      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgga

      901 agattttgtg cgacaatgct tcaatccgat gattgtcgag cttgcggaaa aaacaatgaa

      961 agagtatggg gaggacctga aaatcgaaac aaacaaattt gcagcaatat gcactcactt

     1021 ggaagtatgc ttcatgtatt cagatttcca cttcatcaat gagcaaggcg agtcaataat

     1081 cgtagaactt ggtgatccta atgcactttt gaagcacaga tttgaaataa tcgagggaag

     1141 agatcgcaca atggcctgga cagtagtaaa cagtatttgc aacactacag gggctgagaa

     1201 accaaagttt ctaccagatt tgtatgatta caaggaaaat agattcatcg aaattggagt

     1261 aacaaggaga gaagttcaca tatactatct ggaaaaggcc aataaaatta aatctgagaa

     1321 aacacacatc cacattttct cgttcactgg ggaagaaatg gccacaaagg ccgactacac

     1381 tctcgatgaa gaaagcaggg ctaggatcaa aaccaggcta ttcaccataa gacaagaaat

     1441 ggccagcaga ggcctctggg attcctttcg tcagtccgag agaggagaag agacaattga

     1501 agaaaggttt gaaatcacag gaacaatgcg caagcttgcc gaccaaagtc tcccgccgaa

     1561 cttctccagc cttgaaaatt ttagagccta tgtggatgga ttcgaaccga acggctacat

     1621 tgagggcaag ctgtctcaaa tgtccaaaga agtaaatgct agaattgaac cttttttgaa

     1681 aacaacacca cgaccactta gacttccgaa tgggcctccc tgttctcagc ggtccaaatt

     1741 cctgctgatg gatgccttaa aattaagcat tgaggaccca agtcatgaag gagagggaat

     1801 accgctatat gatgcaatca aatgcatgag aacattcttt ggatggaagg aacccaatgt

     1861 tgttaaacca cacgaaaagg gaataaatcc aaattatctt ctgtcatgga agcaagtact

     1921 ggcagaactg caggacattg agaatgagga gaaaattcca aagactaaaa atatgaaaaa

     1981 aacaagtcag ctaaagtggg cacttggtga gaacatggca ccagaaaagg tagactttga

     2041 cgactgtaaa gatgtaggtg atttgaagca atatgatagt gatgaaccag aattgaggtc

     2101 gcttgcaagt tggattcaga atgagttcaa caaggcatgc gaactgacag attcaagctg

     2161 gatagagctt gatgagattg gagaagatgt ggctccaatt gaacacattg caagcatgag

     2221 aaggaattat ttcacatcag aggtgtctca ctgcagagcc acagaataca taatgaaggg

     2281 ggtgtacatc aatactgcct tacttaatgc atcttgtgca gcaatggatg atttccaatt

     2341 aattccaatg ataagcaagt gtagaactaa ggagggaagg cgaaagacca acttgtatgg

     2401 tttcatcata aaaggaagat cccacttaag gaatgacacc gacgtggtaa actttgtgag

     2461 catggagttt tctctcactg acccaagact tgaaccacac aaatgggaga agtactgtgt

     2521 tcttgagata ggagatatgc ttctaagaag tgccataggc caggtttcaa ggcccatgtt

     2581 cttgtatgtg aggacaaatg gaacctcaaa aattaaaatg aaatggggaa tggagatgag

     2641 gcgttgtctc ctccagtcac ttcaacaaat tgagagtatg attgaagctg agtcctctgt

     2701 caaagagaaa gacatgacca aagagttctt tgagaacaaa tcagaaacat ggcccattgg

     2761 agagtctccc aaaggagtgg aggaaagttc cattgggaag gtctgcagga ctttattagc

     2821 aaagtcggta tttaacagct tgtatgcatc tccacaacta gaaggatttt cagctgaatc

     2881 aagaaaactg cttcttatcg ttcaggctct tagggacaat ctggaacctg ggacctttga

     2941 tcttgggggg ctatatgaag caattgagga gtgcctaatt aatgatccct gggttttgct

     3001 taatgcttct tggttcaact ccttccttac acatgcattg agtggaggcg gcggttccca

     3061 tcaccaccat caccaccatc attgactcga gtctagaggg cccgtttaaa cccgctgatc

     3121 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc

     3181 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc

     3241 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg

     3301 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga

     3361 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt

     3421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc

     3481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca

     3541 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc

     3601 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt

     3661 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac

     3721 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc

     3781 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat

     3841 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc

     3901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga

     3961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc

     4021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt

     4081 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga

     4141 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc

     4201 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac

     4261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca

     4321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt

     4381 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg

     4441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga

     4501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct

     4561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg