• 商品货号：FH1708

• 规格：10μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：博来霉素Zeo
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：          pEF4-V5-HisA是一个哺乳细胞pEF系列表达质粒。
• 质粒图谱1： jpg/1-1G2210Z153116.jpg

质粒序列： 点击查看序列

LOCUS       Exported                5769 bp ds-DNA     circular SYN 21-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5769

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        475..1653

                     /label=EF-1-alpha promoter

                     /note="strong constitutive promoter for human elongation 

                     factor EF-1-alpha"

     promoter        1670..1688

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             1807..1848

                     /codon_start=1

                     /product="epitope tag from simian virus 5"

                     /label=V5 tag

                     /translation="GKPIPNPLLGLDST"

     CDS             1858..1875

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     polyA_signal    1904..2128

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      2174..2602

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2616..2946

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2797..2932

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     promoter        2994..3041

                     /label=EM7 promoter

                     /note="synthetic bacterial promoter "

     CDS             3060..3434

                     /codon_start=1

                     /gene="Sh ble from Streptoalloteichus hindustanus"

                     /product="antibiotic-binding protein"

                     /label=Zeo

                     /note="confers resistance to bleomycin, phleomycin, and 

                     Zeocin(TM)"

                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD

                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR

                     EFALRDPAGNCVHFVAEEQD"

     polyA_signal    3564..3685

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(4136..4724)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4895..5755)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5756..91)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta

       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg

      121 tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga

      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg

      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct

      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca

      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga

      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgcctc gtgaggctcc

      481 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg

      541 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc

      601 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc

      661 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt

      721 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc

      781 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt

      841 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg

      901 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat

      961 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat

     1021 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc

     1081 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg

     1141 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc

     1201 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg

     1261 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg

     1321 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca

     1381 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc

     1441 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt

     1501 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc

     1561 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt

     1621 caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact aatacgactc

     1681 actataggga gacccaagct ggctagttaa gcttggtacc gagctcggat ccactagtcc

     1741 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc

     1801 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat

     1861 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc

     1921 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc

     1981 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct

     2041 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg

     2101 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct

     2161 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg

     2221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct

     2281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta

     2341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt

     2401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg

     2461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat

     2521 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt

     2581 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt

     2641 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc

     2701 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat

     2761 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt

     2821 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc

     2881 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt

     2941 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca

     3001 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca

     3061 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg

     3121 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg

     3181 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca

     3241 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg

     3301 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc

     3361 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg

     3421 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt

     3481 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca

     3541 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa

     3601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt

     3661 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct

     3721 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac

     3781 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac

     3841 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc

     3901 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg

     3961 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc

     4021 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt

     4081 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc

     4141 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa

     4201 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc

     4261 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg

     4321 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc

     4381 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc

     4441 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca

     4501 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact

     4561 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg

     4621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt

     4681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct

     4741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga

    &nb