- 商品货号:FH1707
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pEF1-Myc-HisB是一个哺乳细胞pEF系列表达质粒。
- 质粒图谱1: png/1-1G221092501117.png
质粒序列: 点击查看序列LOCUS Exported 6169 bp ds-DNA circular SYN 21-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..6169/organism="synthetic DNA construct"/mol_type="other DNA"promoter 473..1651/label=EF-1-alpha promoter/note="strong constitutive promoter for human elongationfactor EF-1-alpha"promoter 1668..1686/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"CDS 1806..1835/codon_start=1/product="Myc (human c-Myc proto-oncogene) epitope tag"/label=Myc/translation="EQKLISEEDL"CDS 1851..1868/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 1897..2121/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 2167..2595/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 2609..2938/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 2789..2924/label=SV40 ori/note="SV40 origin of replication"CDS 3005..3799/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 3975..4096/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(4536..5124)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(5295..6155)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(6156..91)/gene="bla"/label=AmpR promoterORIGIN1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag1741 tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc1801 ggttcgaaca aaaactcatc tcagaagagg atctgaatat gcataccggt catcatcacc1861 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat1921 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc1981 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg2041 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg2101 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt2161 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg2221 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc2281 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc2341 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta2401 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta2461 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg2521 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa2581 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg2641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg2701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc2761 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca2821 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc2881 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa2941 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt3001 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct3061 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct3121 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga3181 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc3241 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg3301 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc3361 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca3421 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga3481 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc3541 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga3601 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca3661 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg3721 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct3781 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc3841 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg3901 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt3961 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca4021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac4081 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat4141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac4201 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa4261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat4321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc4381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg4441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag4501 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac4561 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga4621 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt4681 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc4741 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc4801 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta<
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