• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：荧光素酶Luc
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           IgK-IFN-luc是一个人源启动子哺乳细胞荧光素酶报告质粒。
• 质粒图谱1： png/1-1G221144343Y1.png

质粒序列： 点击查看序列

LOCUS       Exported                5863 bp ds-DNA     circular SYN 21-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5863

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      complement(474..1062)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(1233..2093)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(2094..2198)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      complement(2224..2679)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     polyA_signal    2872..3006

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     misc_feature    3023..3180

                     /label=IgK-IFN

     CDS             3210..4862

                     /codon_start=1

                     /gene="luc"

                     /product="firefly luciferase"

                     /label=luciferase

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"

     intron          5086..5151

                     /label=small t intron

                     /note="simian virus 40 (SV40) small t antigen intron"

     CDS             5281..5301

                     /codon_start=1

                     /product="nuclear localization signal of SV40 large T 

                     antigen"

                     /label=SV40 NLS

                     /translation="PKKKRKV"

     polyA_signal    5726..5860

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

ORIGIN

        1 ctcgaggggg ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg

       61 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac

      121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc

      181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg

      241 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct

      301 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac

      361 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga

      421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat

      481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac

      541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct

      601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg

      661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg

      721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt

      781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg

      841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac

      901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga

      961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt

     1021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt

     1081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga

     1141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc

     1201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct

     1261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata

     1321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca

     1381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga

     1441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga

     1501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg

     1561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga

     1621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt

     1681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct

     1741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca

     1801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat

     1861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga

     1921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc

     1981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg

     2041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc

     2101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt

     2161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca

     2221 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct

     2281 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg

     2341 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact

     2401 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac

     2461 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga

     2521 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga

     2581 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca

     2641 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc

     2701 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag

     2761 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt

     2821 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gaacttgttt

     2881 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca

     2941 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc

     3001 tggatcgatc cccccaccgc ggtggcggcc cacagagggg actttccgag agatctacag

     3061 aggggacttt ccgagagcga gcttgggctg caggtcgaga tccgctgaat agagagagga

     3121 ccatctcata taaataggcc atacccatgg agaaaggaca ttctaactgc aacctttcgc

     3181 aagcttggca ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc

     3241 cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga

     3301 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca

     3361 tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg

     3421 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc

     3481 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg

     3541 aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa

     3601 aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta

     3661 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc

     3721 atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa

     3781 caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc

     3841 cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa

     3901 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta

     3961 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag

     4021 agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc

     4081 tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg

     4141 aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct

     4201 tccatcttcc agggatacga caaggatatg ggctcactga gactacatca gctattctga

     4261 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag

     4321 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat

     4381 gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct

     4441 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac

     4501 acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc

     4561 ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag

     4621 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa

     4681 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa

     4741 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg

     4801 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt

     4861 aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta ttgtaatatt atatgcaaat

     4921 tgatgaatgg taattttgta attgtgggtc actgtactat tttaacgaat aataaaatca

     4981 ggtataggta actaaaaagg aattcggggg atctttgtga aggaacctta cttctgtggt

     5041 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt

     5101 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc

     5161 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc

     5221 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct

     5281 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt

     5341 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag