• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              pRL-TK-intronless是一个哺乳海肾荧光素报告质粒，由武汉淼灵生物科技有限公司构建并共享至淼灵质粒平台。
• 质粒图谱1： png/1-1G203095209119.png

质粒序列： 点击查看序列

LOCUS       Exported                3811 bp ds-DNA     circular SYN 03-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..3811

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        8..759

                     /label=HSV TK promoter

                     /note="herpes simplex virus thymidine kinase promoter"

     promoter        772..790

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             800..1735

                     /codon_start=1

                     /product="luciferase from the anthozoan coelenterate 

                     Renilla reniformis (sea pansy)"

                     /label=Rluc

                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN

                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW

                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI

                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE

                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV

                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"

     polyA_signal    1766..1887

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     promoter        2020..2124

                     /gene="bla"

                     /label=AmpR promoter

     CDS             2125..2985

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      3156..3744

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct

       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt

      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc

      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc

      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg

      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg

      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac

      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat

      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat

      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt

      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac

      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt

      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttagtac ttaatacgac

      781 tcactatagg ctagccacca tgacttcgaa agtttatgat ccagaacaaa ggaaacggat

      841 gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg attcatttat

      901 taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac atggtaacgc

      961 ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag cgcggtgtat

     1021 tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt cttataggtt

     1081 acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac caaagaagat

     1141 catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct atgagcatca

     1201 agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg aatcatggga

     1261 tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag gagaaaaaat

     1321 ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt

     1381 agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg aagttcgtcg

     1441 tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt

     1501 acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac caaaaatgtt

     1561 tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca agaagtttcc

     1621 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga

     1681 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataattcta

     1741 gagcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact

     1801 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta

     1861 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag

     1921 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa

     1981 atcgataagg atccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta

     2041 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt

     2101 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc

     2161 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa

     2221 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt

     2281 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt

     2341 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc

     2401 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg

     2461 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg

     2521 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac

     2581 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca

     2641 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta

     2701 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat

     2761 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa

     2821 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag

     2881 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat

     2941 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt

     3001 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg

     3061 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga

     3121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta

     3181 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa

     3241 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact

     3301 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca

     3361 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt

     3421 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg

     3481 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag

     3541 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta

     3601 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat

     3661 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg

     3721 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc

     3781 ttttgctggc cttttgctca catggctcga c

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