• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：绿色荧光蛋白ZsGreeen1
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：             pAAV-ZsGreen1-shRNA是一个腺相关病毒干扰质粒。
• 质粒图谱1： png/8-1P202110103124.png

质粒序列： 点击查看序列

LOCUS       Exported                5753 bp ds-DNA     circular SYN 30-JAN-2018

FEATURES             Location/Qualifiers

     source          1..5753

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        135..438

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        439..642

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             1251..1946

                     /codon_start=1

                     /product="Zoanthus green fluorescent protein"

                     /label=ZsGreen1

                     /note="mammalian codon-optimized"

                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN

                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV

                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG

                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH

                     AIASGSALP"

     polyA_signal    1955..2431

                     /label=hGH poly(A) signal

                     /note="human growth hormone polyadenylation signal"

     promoter        2553..2793

                     /label=U6 promoter

                     /note="RNA polymerase III promoter for human U6 snRNA"

     rep_origin      complement(3057..3645)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3816..4676)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4677..4781)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      complement(5063..5518)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     repeat_region   5593..5733

                     /label=AAV2 ITR

                     /note="inverted terminal repeat of adeno-associated virus 

                     serotype 2"

ORIGIN

        1 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cactagttct

       61 agaacgcgtg gagctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca

      121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac

      181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact

      241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa

      301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg

      361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta

      421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg

      481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg

      541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg

      601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag

      661 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc

      721 agcctccgcg gattcgaatc ccggccggga acggtgcatt ggaacgcgga ttccccgtgc

      781 caagagtgac gtaagtaccg cctatagagt ctataggccc acaaaaaatg ctttcttctt

      841 ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg

      901 caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt

      961 tctgggttaa ggcaatagca atatttctgc atataaatat ttctgcatat aaattgtaac

     1021 tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat tctgctttta

     1081 ttttatggtt gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca

     1141 tgttcatacc tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg

     1201 cccatcactt tggcaaagaa ttgggattcg aacatcgatt gaattccacc atggcccagt

     1261 ccaagcacgg cctgaccaag gagatgacca tgaagtaccg catggagggc tgcgtggacg

     1321 gccacaagtt cgtgatcacc ggcgagggca tcggctaccc cttcaagggc aagcaggcca

     1381 tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc cgaggacatc ttgtccgccg

     1441 ccttcatgta cggcaaccgc gtgttcaccg agtaccccca ggacatcgtc gactacttca

     1501 agaactcctg ccccgccggc tacacctggg accgctcctt cctgttcgag gacggcgccg

     1561 tgtgcatctg caacgccgac atcaccgtga gcgtggagga gaactgcatg taccacgagt

     1621 ccaagttcta cggcgtgaac ttccccgccg acggccccgt gatgaagaag atgaccgaca

     1681 actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa gcagggcatc ttgaagggcg

     1741 acgtgagcat gtacctgctg ctgaaggacg gtggccgctt gcgctgccag ttcgacaccg

     1801 tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg gcacttcatc cagcacaagc

     1861 tgacccgcga ggaccgcagc gacgccaaga accagaagtg gcacctgacc gagcacgcca

     1921 tcgcctccgg ctccgccttg ccctaaagat ctacgggtgg catccctgtg acccctcccc

     1981 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa

     2041 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg

     2101 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg

     2161 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt

     2221 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag

     2281 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt

     2341 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca

     2401 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc

     2461 tcgaaattcc ccagtggaaa gacgcgcagg caaaacgcac cacgtgacgg agcgtgaccg

     2521 cgcgccgagc gcgcgccaag gtcgggcagg aagagggcct atttcccatg attccttcat

     2581 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca

     2641 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag

     2701 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg

     2761 atttcttggg tttatatatc ttgtggaaag gacgcgggat ccgcgagctg caggactcta

     2821 actcgagtta gagtcctgca gctcgctttt taagcttgcg gccgcaggaa cccctagtga

     2881 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg

     2941 tcgcttgacg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggacatg

     3001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc

     3061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga

     3121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct

     3181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg

     3241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag

     3301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat

     3361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac

     3421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac

     3481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc

     3541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt

     3601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc

     3661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg

     3721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca

     3781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca

     3841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag

     3901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac

     3961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc

     4021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct

     4081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc

     4141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg

     4201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc

     4261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat

     4321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag

     4381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat

     4441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg

     4501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca

     4561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga

     4621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc

     4681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata

     4741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg

     4801 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc

     4861 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag

     4921 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag

     4981 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag

     5041 attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa attcgcgtta

     5101 aatttttgtt aaatcagctc attttttaac caatagaccg aaatcggcaa aatcccttat

     5161 aaatcaaaag aatagcccga gatagagttg agtgttgttc cagtttggaa caagagtcca

     5221 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc

     5281 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta

     5341 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg

     5401 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta aggcgctggc aagtgtagcg

     5461 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac

     5521 tatggttgct ttgacgtatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc

     5581 gcatcaggcg cccctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa

     5641 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag

     5701 agggagtggc caactccatc actaggggtt cctgcggccg cactagttct aga

//