• 商品货号：FH1599

• 规格：10μl质粒
• 复制子：pUC ori
• 质粒大小：7750bp
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 备注：高拷贝
• 质粒简介：        pPSU2质粒是一个用于制备DNA Marker的高拷贝质粒，如果用Pst1单酶切，会出现50bp,100bp,200bp,300bp,400bp,500bp,600bp,1500bp,4000bp等条带，如果用EcoRI单酶切，会出现750bp,3000bp,4000bp等条带。
• 质粒图谱1： png/1-1G026144002635.png

质粒序列： 点击查看序列

LOCUS       Exported                7750 bp ds-DNA     circular SYN 26-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7750)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..7750

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        5844..5948

                     /gene="bla"

                     /label=AmpR promoter

     CDS             5949..6809

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      6980..7568

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca

       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct

      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat

      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttga

      241 tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag

      301 tgaatatatc gaacagtcag gttaacctgc agcggcattt tgtccgcgcc gggcttcgct

      361 cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc

      421 cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca

      481 tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg

      541 gttattccaa aatgctgctg ggtgtttatg cctactttat agagcataag cagcgcaaca

      601 cccttatctg gttgccaacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc

      661 cgactattcg tgatattccg tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc

      721 gggataacac gctcaccatg aagcgtttca ctaatgggcg tggcttctgg tgcctgggcg

      781 gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt ggcgggttat gatgaacttg

      841 ctgcttttga tgatgatatt gaacaggaag gctctccgac gttcctgggt gacaagcgta

      901 ttgaaggctc ggtctggcca aagtccatcc gtggctccac gccaaaagtg agaggcacct

      961 gtcagattga gcgtgcagcc agtgaagcga tatcggatct ttccgagcat ttattaagca

     1021 tttcgctata agttctcgct ggaagaggta gttttttcat tgtactttac cttcatctct

     1081 gttcattatc atcgctttta aaacggttcg accttctaat cctatctgac cattataatt

     1141 ttttagaatg gtttcataag aaagctctga atcaacggac tgcgataata agtggtggta

     1201 tccagaattt gtcacttcaa gtaaaaacac ctcacgagtt aaaacaccta agttctcacc

     1261 gaatgtctca atatccggac ggataatatt tattgcttct cttgaccgta ggactttcca

     1321 catgcaggat tttggaacct cttgcagtac tactggggaa tgagttgcaa ttattgctac

     1381 accattgcgt gcatcgagta agtcgcttaa tgttcgtaaa aaagcagaga gcaaaggtgg

     1441 atgcagatga acctctggtt catcgaataa aactaatgac ttttcgccaa cgacatctac

     1501 taatcttgtg atagtaaata aaacaattgc atgtccagag ctcattcgaa gcagatattt

     1561 ctggatattg tcataaaaca atttagtgaa tttatcatcg tccacttgaa tctgtggttc

     1621 attacgtctt aactcttcat atttagaaat gaggctgatg agttccatat ttgaaaagtt

     1681 ttcatcacta cttagttttt tgatagcttc aagccagagt tgtctttttc tatctactct

     1741 catacaacca ataaatgctg aaatgaattc taagcggaga tcgcctagtg attttaaact

     1801 attgctggca gcattcttga gtccaactgc agagtattgt gtaccttttg ctgggtcagg

     1861 ttgttcttta gccggcctgc aggaagagga tggtgcagca accaacaaga aaacactggc

     1921 agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgcctgc

     1981 agtaactttc cccacaacgg aacaactctc attgcatggg atcattgggt actgtgggtt

     2041 tagtggttgt aaaaacacct gaccgctatc cctgatcagt ttcttgaagg taaactcatc

     2101 acccccaagt ctggctatgc agaaatcacc tggctcaaca gcctgctcag ggtcaacgag

     2161 aattaacatt ccgtcactgc agacggctca ggatacggat aacggctact ccgtgtttga

     2221 gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg

     2281 gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta

     2341 ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg

     2401 gctggaagag gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gcttcagttt

     2461 tcaggaagcc agatctctgc aggttgacgg ttttccacca tcgcacgccg ggaccatcac

     2521 cgtgtatgaa gattcacaac cggggacgct gaatgatttt ctctgtgcca tgacggagga

     2581 tgatgcccgg ccggaggtgc tgcgtcgtct tgaactgatg gtggaagagg tggcgcgtaa

     2641 cgcgtccgtg gtggcacaga gtacggcaga cgcgaagaaa tcagccggcg atgccagtgc

     2701 atcagctgct caggtcgcgg cccttgtgac tgatgcaact gactcagcac gcgccgccag

     2761 cacgtccgcc ggacaggctg catcgtcagc tcaggaagcg tcctccggcg cagaagcggc

     2821 atcagcaaag gccactgaag cggaaaaaag tgccgcagcc gcagagtcct caaaaactgc

     2881 aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg acgctgaaaa

     2941 agccgaaaca gtccagcgtg aaggtgtctg cgggcgatcg tcaggaagac agtgctcatg

     3001 ctgccctgct gacgcttcag gcagaactcc ggacgctgga gaagcatgtc ggagcaaatg

     3061 agaaaatcag ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc gcggtactgg

     3121 aggaggcggc gcaacgtcgc cagctgtctg cacaggagaa atccctgctg gcgcataaag

     3181 atgagacgct ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg

     3241 agcgcctgaa cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac

     3301 gggccgccat tgatgcgaaa agccgggggc tgactgaccg gcaggcagaa cgggaagcca

     3361 cggaacagcg cctgaactgc agggatccct tctggggccg gacggcatga catcgctgcg

     3421 cgaatatgcc ggttatcacg gcggtggcag cggatttgga gggcagttgc ggtcgtggaa

     3481 cccaccgagt gaaagtgtgg atgcagccct gttgcccaac tttacccgtg gcaatgcccg

     3541 cgcagacgat ctggtacgca ataacggcta tgccgccaac gccatccagc tgcatcagga

     3601 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat

     3661 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc

     3721 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg

     3781 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac

     3841 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa

     3901 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg

     3961 tgcggcgctg ggacatctgc agtgtacaga tatccaaatg aagccatagg catttgttat

     4021 tttggctctg tcagctgcat aacgccaaaa aatatattta tctgcttgat cttcaaatgt

     4081 tgtattgatt aaatcaattg gatggaattg tttatcataa aaaattaatg tttgaatgtg

     4141 ataaccgtcc tttaaaaaag tcgtttctgc aagcttggct gtatagtcaa ctaactcttc

     4201 tgtcgaagtg atatttttag gcttatctac cagttttaga cgctctttaa tatcttcagg

     4261 aattatttta ttgtcatatt gtatcatgct aaatgacaat ttgcttatgg agtaatcttt

     4321 taattttaaa taagttattc tcctggcttc atcaaataaa gagtcgaatg atgttggcga

     4381 aatcacatcg tcacccattg gattgtttat ttgtatgcca agagagttac agcagttata

     4441 cattctgcca tagattatag ctaaggcatg taataattcg taatctttta gcgtattagc

     4501 gacccatcgt ctttctgatt taataataga tgattcagtt aaatatgaag gtaatttctt

     4561 ttgtgcaagt ctgactaact tttttatacc aatgtttaac atactttcat ttgtaataaa

     4621 ctcaatgtca ttttcttcaa tgtaagatga aataagagta gcctttgcct cgctatacat

     4681 ttctaaatcg ccttgttttt ctatcgtatt gcgagaattt ttagcccaag ccattaatgg

     4741 atcatttttc catttttcaa taacattatt gttataccaa atgtcatatc ctataatctg

     4801 gtttttgttt ttttgaataa taaatgttac tgttcttgcg gtttggagga attgattcaa

     4861 attcaagcga aataattcag ggtcaaaata tgtatcaatg cagcatttga gcaagtgcga

     4921 taaatcttta agtcttcttt cccatggttt tttagtcata aaactctcca ttttgatagg

     4981 ttgcatgcta gatgctgata tattttagag gtgataaaat taactgctta actgtcaatg

     5041 taatacaagt tgtttgatct ttgcaatgat tcttatcaga aaccatatag taaattagtt

     5101 acacaggaaa tttttaatat tattattatc attcattatg tattaaaatt agagttgtgg

     5161 cttggctctg ctaacacgtt gctcatagga gatatggtag agccgcagac acgtcgtatg

     5221 caggaacgtg ctgtggctgg ctggtgaact tccgatagtg cgggtgttga atgatttcca

     5281 gttgctaccg attttacata ttttttgcat gagagaattt gtaccacctc ccaccgacca

     5341 tctatgagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta

     5401 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg

     5461 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc

     5521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta

     5581 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg

     5641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg

     5701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc

     5761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag

     5821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt

     5881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa

     5941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt

     6001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt

     6061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt

     6121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg

     6181 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga

     6241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa

     6301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga

     6361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa

     6421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca

     6481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta

     6541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac

     6601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc

     6661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag

     6721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga

     6781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt

     6841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata

     6901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag

     6961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa

     7021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt

     7081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc

     7141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa

     7201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa

     7261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc

     7321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa

     7381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa

     7441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg