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| 发货规格 |
1×10⁶ |
| 别名 |
SK-Mel-28; SK.MEL.28; SK-MEL 28; SK MEL-28; SK MEL 28; SK Mel 28; SKMel-28; SKMEL-28; SK-MEL28; SK-Mel28; SK Mel28; SKMEL28; SKMel28; SKmel28; SKML-28; SK28; AU-Mel; P-36; P36 |
| 形态 |
多角形 |
| 生长特性 |
贴壁 |
| 培养条件 |
RPMI-1640+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
27h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
皮肤 |
| 肿瘤类型 |
皮肤黑色素瘤 |
| 供体信息 |
51岁男性 |
| 背景描述 |
该细胞系是由 T.Takahashi 及其同事从一名种族不明的 51 岁男性的腋窝淋巴结中建立的，他们在一系列黑色素瘤系中分离出该细胞系。 |
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| 蛋白表达 |
p53 positive |
| 同工酶 |
PGM3， 1， PGM1， 1， ES-D， 1， AK-1， 1-2， GLO-1， 2， G6PD， B |
| 致瘤性 |
Yes， in nude mice. Forms malignant melanoma (large round cell type) |
| 抗原表达 |
Blood Type A; Rh+; HLA A11， A26， B40， DRw4 |
| 突变信息 |
BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing， RT-PCR) and protein based methods (Western Blot)， N-Ras wt |
| 核型 |
modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90， occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21)， del(1) (q2101) and several others had two copies per cell and t(2p14q)， t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y， N1 and N11 were absent; N19 had five or more copies per cell. |
