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| 发货规格 |
1×10⁶ |
| 别名 |
MDA-MB 468; MDA-MB468; MDAMB468; MDA-468; MDA468; MB468; MD Anderson-Metastatic Breast-468 |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
L15+10%FBS+1%P/S+10ug/ml胰岛素+15ug/ml谷胱甘肽 |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
30~40h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
乳腺 |
| 肿瘤类型 |
乳腺癌 |
| 供体信息 |
51岁黑人女性 |
| 背景描述 |
成立于1977年，由一名51岁浸润性乳腺癌女性的胸腔积液组成;文献中描述细胞在密码子273处携带p53点突变，导致Arg-His取代和扩增的EGFR;三阴性 |
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| 蛋白表达 |
Epidermalgrowthfactor(EGF);transforminggrowthfactoralpha(TGFalpha)。蛋白质印迹中无ER、PR、AR和HER2证实三阴性 |
| 同工酶 |
AK-1，1；ES-D，1；G6PD，A；GLO-I，1-2；Me-2，1-2；PGM1，1；PGM3，2 |
| 致瘤性 |
Yes， in nude mice inoculated subcutaneously with 10(7) cells.(Tumors developed within 21 days at 100% frequency (5/5).) |
| 抗原表达 |
Blood Type AB;HLA Aw23，Aw30，B27，Bw35，Cw2，Cw4 (patient) |
| 病毒 |
EBV-，HBV-，HCV-，HIV-1-，HIV-2-，HTLV-I/-II-，MLV- |
| 核型 |
modal number = 64; range = 60 to 67.The cell line is aneuploid human， presumably female (X， abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X， N2， N3， N7， N8， N10， and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype， but， in addition， regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype. |
