• 靶点-Target： PKM2
• MW(Observed)： 60kD
• 宿主物种-Host Species： Mouse
• 修饰-Modification： Unmodified
• 推荐稀释比： WB 1:1000;ICC 1:400;IF 1:50-200
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 克隆性： Monoclonal
• 免疫原： Purified recombinant human PKM2 protein fragments expressed in E.coli.
• 特异性： This antibody detects endogenous levels of PKM2 and does not cross-react with related proteins.
• 基因名称： pkm2
• Organism-1： Human
• 基因ID-1： 5315
• SwissProt-1： P14618
• Organism-2： Mouse
• SwissProt-2： P52480
• 背景： This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP， generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein， a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells， suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq， May 2011]，
• 细胞定位： [Isoform M2]: Cytoplasm . Nucleus . Translocates to the nucleus in response to various signals， such as EGF receptor activation or apoptotic stimuli (PubMed:17308100， PubMed:22056988， PubMed:24120661). Nuclear translocation is promoted by acetylation by EP300 (PubMed:24120661). Deacetylation by SIRT6 promotes its nuclear export in a process dependent of XPO4， thereby suppressing its ability to activate transcription and promote tumorigenesis (PubMed:26787900). .; [Isoform M1]: Cytoplasm .

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