• 靶点-Target： PRK2
• MW(Calculated)： 112kD
• 宿主物种-Host Species： Mouse
• 修饰-Modification： Unmodified
• 推荐稀释比： WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： Affinity purification
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 克隆性： Monoclonal
• 免疫原： Purified recombinant fragment of human PRK2 expressed in E. Coli.
• 特异性： PRK2 Monoclonal Antibody detects endogenous levels of PRK2 protein.
• 基因名称： PKN2
• 蛋白名称： Serine/threonine-protein kinase N2
• Organism-1： Human
• 基因ID-1： 5586
• SwissProt-1： Q16513
• Organism-2： Mouse
• 基因ID-2： 109333
• SwissProt-2： Q8BWW9
• Organism-3： Rat
• SwissProt-3： O08874
• 背景： catalytic activity:ATP + a protein = ADP + a phosphoprotein.，domain:The C1 domain does not bind the diacylglycerol (DAG).，enzyme regulation:Activated by lipids， particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites， Thr-816 (activation loop of the kinase domain) and Thr-958 (turn motif)， need to be phosphorylated for its full activation.，function:Exhibits a preference for highly basic protein substrates.，PTM:Activated by limited proteolysis with trypsin.，PTM:Autophosphorylated.，similarity:Belongs to the protein kinase superfamily.，similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.，similarity:Contains 1 AGC-kinase C-terminal domain.，similarity:Contains 1 C2 domain.，similarity:Contains 1 protein kinase domain.，similarity:Contains 3 REM (Hr1) repeats.，
• 细胞定位： Cytoplasm . Nucleus . Membrane . Cell projection， lamellipodium . Cytoplasm， cytoskeleton . Cleavage furrow . Midbody . Cell junction . Colocalizes with PTPN13 in lamellipodia-like structures， regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection， colocalizes with HCV NS5B at perinuclear region in the cytoplasm. .

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