• 靶点-Target： NFkB p65
• MW(Observed)： 65kD
• 宿主物种-Host Species： Rabbit
• 同种型-Isotype： IgG
• 修饰-Modification： Acetyl
• 推荐稀释比： WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 浓度： 1 mg/ml
• 克隆性： Polyclonal
• 免疫原： Synthesized acetyl-peptide derived from the Internal region of human NFκB-p65 around the acetylation site of K221.
• 特异性： Acetyl-NFκB-p65 (K221) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylation at K221.
• 基因名称： RELA
• 蛋白名称： Transcription factor p65
• Organism-1： Human
• 基因ID-1： 5970
• SwissProt-1： Q04206
• Organism-2： Mouse
• 基因ID-2： 19697
• SwissProt-2： Q04207
• 背景： NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor， NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL， RELA， or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene， RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq， Sep 2011]，
• 细胞定位： Nucleus . Cytoplasm . Nuclear， but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .

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