• 靶点-Target： p53
• MW(Observed)： 53kD
• 宿主物种-Host Species： Rabbit
• 同种型-Isotype： IgG
• 修饰-Modification： Acetyl
• 推荐稀释比： WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 浓度： 1 mg/ml
• 克隆性： Polyclonal
• 免疫原： The antiserum was produced against synthesized Acetyl-peptide derived from human p53 around the Acetylation site of Lys372. AA range:331-380
• 特异性： Acetyl-p53 (K372) Polyclonal Antibody detects endogenous levels of p53 protein only when acetylated at K372.
• 基因名称： TP53
• 蛋白名称： Cellular tumor antigen p53
• Organism-1： Human
• 基因ID-1： 7157
• SwissProt-1： P04637
• Organism-2： Mouse
• SwissProt-2： P02340
• 背景： tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation， DNA binding， and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes， thereby inducing cell cycle arrest， apoptosis， senescence， DNA repair， or changes in metabolism. Mutations in this gene are associated with a variety of human cancers， including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546， 20937277). [provided by RefSeq， Feb 2013]，
• 细胞定位： Cytoplasm . Nucleus . Nucleus， PML body . Endoplasmic reticulum . Mitochondrion matrix . Cytoplasm， cytoskeleton， microtubule organizing center， centrosome . Recruited into PML bodies together with CHEK2 (PubMed:12810724). Translocates to mitochondria upon oxidative stress (PubMed:22726440). Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408). .; [Isoform 1]: Nucleus . Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.; [Isoform 2]: Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 3]: Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells.; [Isoform 4]: Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.; [Isoform 7]: Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 8]: Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells， forms foci in the nucleus that are different from nucleoli.; [Isoform 9]: Cytoplasm.

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