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| 发货规格 |
1×10⁶ |
| 别名 |
H520; H-520; NCI-HUT-520; NCIH520 |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
RPMI-1640+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
肺 |
| 肿瘤类型 |
鳞状细胞癌 |
| 背景描述 |
该细胞系于 1982 年从 A.F. Gazdar 从肺鳞状细胞癌患者身上采集的肺肿块样本中建立。与正常肺组织相比，该细胞系表达的 p53 mRNA 水平大大降低。这些细胞没有表现出明显的结构DNA异常。细胞的角蛋白和波形蛋白染色呈阳性，但神经丝三联体蛋白呈阴性。细胞可以在有/没有血清的软琼脂中形成集落。 |
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| 蛋白表达 |
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. |
| 同工酶 |
AK-1， 1；ES-D， 1；G6PD， B；GLO-I， 2；Me-2， 0；PGM1， 1；PGM3， 1 |
| 基因表达 |
The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue， but exhibit no gross structural DNA abnormalities. |
| 致瘤性 |
Yes， in nude mice inoculated subcutaneously with 1*10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)). |
| 核型 |
This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells， and four others were found in some cells. Among the common markers were 1q+， t(1q8q)， 2q+， der(16)t(3;16)(q21;q22)， der(19)t(13;19)(q21;q13)， and der(5)t(5;5)(p15pq13). Generally， there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent， and the X chromosome was single. |
