• 规格：20μl质粒
• 质粒分类：广宿主载体
• 质粒大小：5996 bp
• 质粒标签：EGFP
• 原核抗性：Kan（50μg/ml）
• 克隆菌株：DH5α
• 表达宿主：革兰氏阴性菌

质粒序列： 点击查看序列

accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc 61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg 121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg 181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc 421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta 1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga 1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat 1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc 1801 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacc gcggaacccc 1861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 1921 ataaatgctt caataatatt gaaaaaggaa gagtatggtg agcaagggcg aggagctgtt 1981 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 2041 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 2101 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 2161 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 2221 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 2281 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 2341 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 2401 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 2461 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 2521 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 2581 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 2641 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc 2701 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aatctagagc 2761 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac 2821 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 2881 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 2941 cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt 3001 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgactgcga 3061 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg 3121 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg 3181 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta 3241 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt 3301 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc 3361 agagcctgat aaaaacggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga 3421 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc 3481 gaggcggcta cagccgatag tctggaacag cgcacttacg ggttgctgcg caacccaagt 3541 gctaccggcg cggcagcgtg acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa 3601 aacacggctc atcgggcatc ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt 3661 cggtcaaggc tggcaggtct ggttccatgc ccggaatgcc gggctggctg ggcggctcct 3721 cgccggggcc ggtcggtagt tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt 3781 cgccatgccc caacagcgat tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga 3841 acaccgacag gcgcaactgg tcgcggggct ggccccacgc cacgcggtca ttgaccacgt 3901 aggccgacac ggtgccgggg ccgttgagct tcacgacgga gatccagcgc tcggccacca 3961 agtccttgac tgcgtattgg accgtccgca aagaacgtcc gatgagcttg gaaagtgtct 4021 tctggctgac caccacggcg ttctggtggc ccatctgcgc cacgaggtga tgcagcagca 4081 ttgccgccgt gggtttcctc gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg 4141 tttgcaccca gtgaccgggc ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg 4201 ccatgcttat ctccatgcgg tagggtgccg cacggttgcg gcaccatgcg caatcagctg 4261 caacttttcg gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga 4321 ttttctttaa cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc 4381 cccctttttt aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt 4441 ggtgcccaaa gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc 4501 cggcaaaaag tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca 4561 aggtggcgct gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg 4621 ggcgggcttc gccttcgact gcccccactc gcataggctt gggtcgttcc aggcgcgtca 4681 aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact tggtgtccaa 4741 ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa 4801 attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct 4861 gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag 4921 cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg 4981 ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc 5041 ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt gcgaagcaaa 5101 gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt gcgctgcccc 5161 cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg cgatcatgcg 5221 atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg cctaccgcga 5281 gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact gggcggccag 5341 cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc ggcgcaagga 5401 cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga agtcggccag 5461 ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg acaagtacgg 5521 ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc acatgaccgc 5581 gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca tcggcaacaa 5641 agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc tagggctgca 5701 acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct acgaggccct 5761 ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc cacgcgccta 5821 tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga cgccggaagc 5881 cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc tacaggccgc 5941 cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg cccgag