• 规格：20μl质粒
• 启动子：T7/lac promoter
• 质粒分类：大肠敲除质粒
• 质粒大小：5634 bp
• 质粒标签：N-Strep-Tag II，N-HRV 3C site
• 原核抗性：壮观霉素Spe
• 筛选标记：
• 克隆菌株：Stbl3

质粒序列： 点击查看序列

ggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatgtcaagctggagccacccgcagttcgaaaagggtgcacttgaagtcctctttcagggacccgggtaccagatgaaccaccttggaaaaacggaagtgttcctaaaccgcttcgccctacggcctctgaaccccgaggaactcaggccttggcgcctcgaggtggttctggaccctcctccggggcgggaggaggtgtatccccttctcgcccaggtggcccgccgggcggggggcgtcacggtgcgcatgggagacggcctcgcctcctggtcaccccctgaggtcctggtcttggagggcaccttggcgcggatggggcagaactacgcctaccgcctctaccccaaggggaggaggcctctggaccccaaggacccgggggagcggagcgtgctttcggccctagcccgaaggctccttcaggagcgcctcaggcgcctcgagggggtctgggtggaggggcttgcggtgtaccggagggagcacgcccgggggcccgggtggcgggtgcttgggggggcggtcttggacctttgggtctccgactcgggggcgttcctcctggaggtggaccccgcttaccgcatcctctgcgagatgtccctcgaggcctggcttgcccaaggccaccctctgcccaaacgggtccggaacgcctacgaccggcgcacctgggagcttctccggctgggagaggaagaccccaaggagctccctctcccgggcggcttgagcctcctggactaccacgctttcaagggccgcctccagggccgggaaggggggcgggtggcctgggtggcggacccaaaggatccgcgaaagccaatcccccatctgacgggccttttggtgcccgttctgaccctggaagaccttcatgaagaggagggaagcctggccctctccttgccttgggaagagcggcgtcggcggacccgggagatcgccagctggatcggccggcgcctggggctgggcacacctgaggcggtgcgcgcccaggcttaccgcttgagcatccccaagctcatgggcagaagagccgtgagcaagcctgcggacgccctccgcgtggggttttaccgggcccaggaaaccgccctggccctcttgcgcctggacggggcccaaggatggccggagtttctccggcgggccttgctccgggcctttggggcgagcggggcttccctccgtttgcacaccctccacgcccatccctcccaaggcctggctttccgcgaggcgctgcggaaggccaaggaggagggggtccaggccgtgctggtcctcaccccgcccatggcctgggaagaccgcaaccgcctgaaggcccttcttctcagggaaggccttcccagccaaatcctcaacgtccccctccgggaggaggaacgccaccgctgggagaacgccctcctgggcctcctggccaaagcggggcttcaggtggtggccctgagcggcgcctatccggcggagctcgccgtgggctttgacgccggcggaagggagtcctttcgcttcgggggcgcggcctgcgccgtgggcggggacggcggccacctcctctggaccctccccgaggcccaggccggggagcggatcccccaggaggtggtctgggacctcttggaggagaccctctgggccttcagacgcaaggcggggaggcttccttcccgggtcctcctccttcgggacggccgcgtgccccaggacgagttcgccctggccttggaggcccttgcccgggaaggcatagcctacgacttggtttcggtgcgcaagtcgggtggggggcgggtctaccccgtgcaggggcgcctggcggacgggctttacgtccccttggaggacaagacctttcttctccttaccgtccaccgggacttccggggcacgccccgacccctgaagctggtgcacgaggcgggggacacgcccctcgaggccctggcccaccagattttccatctgacccgcctctacccggcgagcggtttcgccttcccccggcttcccgctccccttcacctggccgaccgcctggtgaaggaggtgggccggttggggatccgtcacctcaaggaggtggaccgggaaaagctcttcttcgtttaggaattcgcggccgcagagctcgctctggtgccacgcggtagttccgctcatcaccaccatcatcaccatcaccaccactaattaacctaggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaacctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcatcgtggccggatcttgcggcccctcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagctagctcactcggtcgctacgctccgggcgtgagactgcggcgggcgctgcggacacatacaaagttacccacagattccgtggataagcaggggactaacatgtgaggcaaaacagcagggccgcgccggtggcgtttttccataggctccgccctcctgccagagttcacataaacagacgcttttccggtgcatctgtgggagccgtgaggctcaaccatgaatctgacagtacgggcgaaacccgacaggacttaaagatccccaccgtttccggcgggtcgctccctcttgcgctctcctgttccgaccctgccgtttaccggatacctgttccgcctttctcccttacgggaagtgtggcgctttctcatagctcacacactggtatctcggctcggtgtaggtcgttcgctccaagctgggctgtaagcaagaactccccgttcagcccgactgctgcgccttatccggtaactgttcacttgagtccaacccggaaaagcacggtaaaacgccactggcagcagccattggtaactgggagttcgcagaggatttgtttagctaaacacgcggttgctcttgaagtgtgcgccaaagtccggctacactggaaggacagatttggttgctgtgctctgcgaaagccagttaccacggttaagcagttccccaactgacttaaccttcgatcaaaccacctccccaggtggttttttcgtttacagggcaaaagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctactgaaccgctctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgtaattctcatgttagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaaattaatacgactcactata