• 规格：20μl质粒
• 启动子：CMV
• 质粒分类：哺乳细胞载体
• 质粒大小：5508 bp
• 质粒标签：N-3×FLAG
• 原核抗性：Amp
• 克隆菌株：DH5α

质粒序列： 点击查看序列

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccac tagtccagtg 1021 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt 1081 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 1141 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 1201 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 1261 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 1321 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 1381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 1441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 1501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 1561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 1621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 1681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 1741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 1801 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 1861 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 1921 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 1981 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2041 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 2101 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 2161 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 2221 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 2281 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 2341 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 2401 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 2461 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 2521 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 2581 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 2641 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 2701 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 2761 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 2821 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 2881 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 2941 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 3001 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 3061 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 3121 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 3181 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3241 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 3301 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 3361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 3421 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 3481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 3541 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 3601 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 3661 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 3721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 3781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 3841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 3901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 3961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 4081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 4141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 4201 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 4261 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga 4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 5041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 5101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 5161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 5221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 5281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc