• 商品货号：BR405

• 规格：10μl质粒
• 启动子：gpdA,TrpC
• 复制子：pUC ori
• 终止子：trpC
• 质粒分类：其他宿主穿梭载体；丝状真菌表达质粒
• 质粒大小：6226 bp
• 质粒标签：EGFP
• 原核抗性：Amp
• 筛选标记：草胺膦Bar
• 克隆菌株：DH5α
• 表达宿主：丝状真菌

质粒序列： 点击查看序列

1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcttactt 61 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat 121 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt 181 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag 241 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt 301 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc 361 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt 421 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat 481 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat 541 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg 601 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt 661 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag 721 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggatcc 781 tctagagtcg aggtcgacgg tatcgataag cttgattaaa ggttcttgga tgggaagatg 841 aatatactga agatgggaaa agaaagagaa aagaaaagag cagctggtgg ggagagcagg 901 aaaatatggc aacaaatgtt ggactgacgc aacgaccttg tcaaccccgc cgacacaccg 961 ggcggacaga cggggcaaag ctgcctacca gggactgagg gacctcagca ggtcgagtgc 1021 agagcaccgg atgggtcgac tgccagcttg tgttcccggt ctgcgccgct ggccagctcc 1081 tgagcggcct ttccggtttc atacaccggg caaagcagga gaggcacgat atttggacgc 1141 cctacagatg ccggatgggc caattaggga gcttacgcgc cgggtactcg ctctacctac 1201 ttcggagaag gtactatctc gtgaatcttt taccagatcg gaagcaattg gacttctgta 1261 cctaggttaa tggcatgcta tttcgccgac ggctatacac ccctggcttc acattctcct 1321 tcgcttactg ccggtgattc gatgaagctc catattctcc gatgatgcaa tagattcttg 1381 gtcaacgagg ggcacaccag cctttccact tcggggcgga ggggcggccg gtcccggatt 1441 aataatcatc cactgcacct cagagccgcc agagctgtct ggcgcagtgg cgcttattac 1501 tcagcccttc tctctgcgtc cgtccgtctc tccgcatgct ttgcatactt ctgcctgctg 1561 gggagcctgg ggactttcca caccctaact gacacacatt ccacagagat ctctgcctcg 1621 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 1681 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 1741 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct 1801 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 1861 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga 1921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 1981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 2101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 2161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 2221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 2281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 2341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 2401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 2461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 2521 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 2581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 2641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 2701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 2761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 2821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 2881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 2941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 3001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 3061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 3181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 3241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 3301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 3361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 3421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 3481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 3541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 3601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 3661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 3721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 3781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 3841 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 3901 tcaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga 3961 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt 4021 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag 4081 gccgcagaat gtgctctaga ctcgacagaa gatgatattg aaggagcact ttttgggctt 4141 ggctggagct agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg 4201 agcacaaaat ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc 4261 cacttgtagc agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg 4321 acattattat catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa 4381 gtgaacgacc cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat 4441 ctgacttacc tattctaccc aagcaaagct tcgattagga agtaaccatg agcccagaac 4501 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg 4561 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg 4621 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg 4681 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact 4741 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca 4801 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg 4861 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc 4921 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc 4981 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgat 5041 ccgtcgacct gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt 5101 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat 5161 taacatgtaa tgcatagtac cgagaaacta gtggatctaa acataaaatc tgtaaaataa 5221 caagatgtaa agataatgct aaatcatttg gctttttgat tgattgtaca ggaaaatata 5281 catcgcaggg ggttgacttt taccatttca ccgcaatgga atcaaacttg ttgaagagaa 5341 tgttcacagg cgcatacgct acaatgaccc gattcttgct agccttttct cggtcttgca 5401 aacaaccgcc gcggccggtc gagggaccta ataacttcgt atagcataca ttatacgaag 5461 ttatattaag ggttccggat cgcggccgct cgacctgcag ccaagctagc ttggctggac 5521 gtaaactcct cttcagacct aataacttcg tatagcatac attatacgaa gttatattaa 5581 gggttattga atatgatcgg aattggtcga tcaggtattg ctgttatctg atgagtatac 5641 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac 5701 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt 5761 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc ttgcaaatta 5821 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt 5881 acacgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca 5941 taaaaaccca tctcataaat aacgtcatgc attgcagatg agctgtatct ggaagaggta 6001 aacccgaaac gcgttttatt cttgttgaca tggagctatt aaatcactag aaggcactct 6061 ttgctgcttg gacaaatgaa cgtatcttat cgagatcctg aacaccattt gtctcaactc 6121 cggagctgac atcgacacca acgatcttat atccagattc gtcaagctgt ttgatgattt 6181 cagtaacgtt aagtggatct cggtgacggg caggaccgga cggggc