• 规格：20μl质粒
• 启动子：CMV
• 质粒分类：RNAi系统
• 质粒大小：7891 bp
• 原核抗性：Amp
• 筛选标记：PURO
• 克隆菌株：XL1 Blue

质粒序列： 点击查看序列

1 gacgaaagat tggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 61 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 241 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 301 cttttttgga ggcctaggct tttgcaaaaa gctagcttgc atgcctgcag gtcggccgcc 361 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc 421 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc 481 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc 541 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 601 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 661 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 721 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 781 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 841 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 901 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 961 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 1021 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc 1081 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac 1141 tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 1201 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 1261 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 1321 catttttttc actgcaatct cgtgatacgc ctatttttat aggttaatgt catgataata 1381 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 1441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 1501 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 1561 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 1621 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 1681 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 1741 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 1801 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 1861 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 1921 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 1981 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 2041 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 2101 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 2161 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 2221 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 2281 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 2341 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 2401 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 2461 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 2521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 2581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttt tttgccggat 2641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 2701 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 2761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 2821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 2881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 2941 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 3001 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 3061 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 3121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 3181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 3241 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 3301 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 3361 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 3421 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 3481 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 3541 agctatgaca tgattacgaa ttgcaacgat ttaggtgaca ctatagaaga gaaggaatta 3601 atacgactca ctatagggag agagagagaa ttaccctcac taaagggagg agaagcatga 3661 attcaaggta ccagatctta gttattaata gtaatcaatt acggggtcat tagttcatag 3721 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 3781 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 3841 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 3901 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 3961 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 4021 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 4081 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 4141 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 4201 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg 4261 tggatcctca ccatgtcgtt tactttgacc aacaagaacg tgattttcgt tgccggtctg 4321 ggaggcattg gtctggacac cagcaaggag ctgctcaagc gcgatcccgt cgttttacaa 4381 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 4441 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 4501 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc 4561 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg 4621 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg 4681 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc 4741 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg 4801 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac 4861 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt 4921 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc 4981 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat 5041 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac 5101 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg 5161 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt 5221 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg 5281 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc 5341 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga 5401 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg 5461 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat 5521 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa 5581 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg 5641 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt 5701 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat 5761 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac 5821 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc 5881 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg 5941 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac 6001 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat 6061 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt 6121 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg 6181 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc 6241 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg 6301 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc 6361 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg 6421 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg 6481 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc 6541 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt 6601 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat 6661 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac 6721 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg 6781 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat 6841 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc 6901 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg 6961 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca 7021 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc 7081 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg 7141 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc 7201 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat 7261 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg 7321 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattacagct gagcgccggt 7381 cgctaccatt accagttggt ctggtgtcaa aaataataat aaccaagctt aataaaggat 7441 cttttatttt cattggatct gtgtgttggt tttttgtatg cggccgctag cttggcactg 7501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 7561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 7621 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 7681 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 7741 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 7801 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 7861 aggttttcac cgtcatcacc gaaacgcgcg a