• 规格：20μl质粒
• 启动子：T7
• 质粒分类：大肠杆菌表达载体
• 质粒大小：5900 bp
• 质粒标签：thioredoxin (N端); His (中间和C端)
• 原核抗性：Amp
• 克隆菌株：DH5α
• 备注：pET32a载体用于表达可溶性，具有活性功能的蛋白;pET-32a(+)载体具有N端Thrombin（凝血酶）酶切位点，N端肠激酶酶切位点。pET32a，pET32b，pET32c载体的差异主要是在于多克隆位点，其他地方完全一致。

质粒序列： 点击查看序列

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc gtcggtaccc 241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca 301 gaaccgcgtg gcaccagacc agaagaatga tgatgatgat ggtgcatatg gccagaacca 361 gaaccggcca ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcacccact 421 ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca 481 tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc 541 tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac 601 tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg 661 tcgtcagtca ggtgaataat tttatcgctc atatgtatat ctccttctta aagttaaaca 721 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta 781 atttcgcggg atcgagatcg atctcgatcc tctacgccgg acgcatcgtg gccggcatca 841 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 901 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 961 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 1021 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 1081 gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc 1141 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca 1201 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 1261 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 1321 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 1381 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 1441 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 1501 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 1561 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 1621 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 1681 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 1741 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 1801 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 1861 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 1921 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga catctcggta 1981 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 2041 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 2101 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 2161 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 2221 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct 2281 cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc 2341 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 2401 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 2461 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 2521 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 2581 cggcatggcg gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg 2641 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag 2701 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg 2761 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg 2821 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg 2881 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc 2941 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct 3001 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc 3061 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt 3121 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc 3181 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg 3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 3421 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga 3481 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 3781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 3841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 3901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 3961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4081 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4741 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 4801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 4861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 4921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 4981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 5341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5401 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa 5461 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 5521 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 5581 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 5641 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 5701 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 5761 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 5821 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 5881 agggcgcgtc ccattcgcca