- 商品货号:FH1722
- 规格:10μl质粒
- 启动子:CMV
- 复制子:pUC
- 终止子:WPRE
- 原核抗性:氨苄青霉素Amp
- 筛选标记:嘌呤霉素Puro
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: pLVML-3×FLAG-N6AMT1-IRES-Puro(NM_013240.5)是一个人源基因慢病毒哺乳细胞过表达质粒。
- 质粒图谱1: png/1-1G2131F420925.png
质粒序列: 点击查看序列LOCUS Exported 8840 bp ds-DNA circular SYN 13-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..8840/organism="synthetic DNA construct"/mol_type="other DNA"LTR 1..634/label=3' LTR/note="3' long terminal repeat (LTR) from HIV-1"misc_feature 681..806/label=HIV-1 Psi/note="packaging signal of human immunodeficiency virustype 1"misc_feature 1303..1536/label=RRE/note="The Rev response element (RRE) of HIV-1 allows forRev-dependent mRNA export from the nucleus to thecytoplasm."misc_feature 2028..2143/label=cPPT/CTS/note="central polypurine tract and central terminationsequence of HIV-1 (lacking the first T)"enhancer 2201..2504/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 2505..2708/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"regulatory 2809..2814/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"CDS 2818..2883/codon_start=1/product="three tandem FLAG(R) epitope tags, followed by anenterokinase cleavage site"/label=3xFLAG/translation="DYKDHDGDYKDHDIDYKDDDDK"CDS 2890..3534/codon_start=1/product="pLVML-3×FLAG-N6AMT1-IRES-Puro(NM_013240.5)"/label=N6AMT1/translation="MAGENFATPFHGHVGRGAFSDVYEPAEDTFLLLDALEAAAAELAGVEICLEVGSGSGVVSAFLASMIGPQALYMCTDINPEAAACTLETARCNKVHIQPVITDLVKGLLPRLTEKVDLLVFNPPYVVTPPQEVGSHGIEAAWAGGRNGREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTTALSRQAGQETLSVLKFTKS"misc_feature 3543..4116/label=IRES/note="internal ribosome entry site (IRES) of theencephalomyocarditis virus (EMCV)"CDS 4149..4748/codon_start=1/gene="pac from Streptomyces alboniger"/product="puromycin N-acetyltransferase"/label=PuroR/note="confers resistance to puromycin"/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"misc_feature 4762..5350/label=WPRE/note="woodchuck hepatitis virus posttranscriptionalregulatory element"LTR 5557..6190/label=3' LTR/note="3' long terminal repeat (LTR) from HIV-1"rep_origin complement(6720..7308)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(7479..8339)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(8340..8444)/gene="bla"/label=AmpR promoterpolyA_signal 8492..8626/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"ORIGIN1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggac2821 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac2881 aagctcgaga tggcagggga gaacttcgct acgccgttcc acgggcacgt gggccgcggc2941 gccttcagcg acgtgtacga gcccgcggag gacacgtttc tgcttttgga cgcgctggag3001 gcagcggctg ccgaactggc aggagtggaa atatgcctgg aagtagggtc agggtctggt3061 gtagtatctg cattcctagc ctctatgata ggccctcagg ctttgtacat gtgcactgat3121 atcaaccctg aggcagcagc ttgtacccta gagacagcac gctgtaacaa agttcacatt3181 caaccagtta ttacagattt ggtcaaaggc ttgctaccaa gattgaccga aaaagttgat3241 cttctggtgt ttaatccccc ctatgtagtg actccacctc aagaggtagg aagtcacgga3301 atagaggcag cttgggctgg tggcagaaat ggtcgggaag tcatggacag gttttttccc3361 ctggttccag atctcctttc accaagagga ttattctatt tagttaccat taaagaaaac3421 aacccagaag aaattttgaa aataatgaag acaaaaggtc tgcaaggaac cactgcactt3481 tccagacaag caggccaaga aactctttca gtcctcaagt tcaccaagtc ttagggatcc3541 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg服务时间:24小时在线客服
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