• 规格：20μl质粒
• • 质粒宿主：  枯草杆菌
  • 质粒用途：  蛋白表达，分泌表达，枯草游离质粒；枯草胞内质粒
  • 片段类型：  ORF
  • 片段物种：  空载体
  • 原核抗性：  Amp
  • 筛选标记：  
  • 荧光标记：  
  • 启动子:  Hpall
  • 复制子:  pUC ori，F1 ori
  • 拷贝数:  
  • 感受态:  DH5a
  • 温度:  37度
  • 背景载体:  
  • 正向引物:  PMA0911F：gcgaaaatgcctcacatttgtgcc
  • 反向引物:  PMA0911R：cgggatctcagatctggtacgtacc
  • 诱导方式:
•  

质粒序列： 点击查看序列

LOCUS       Exported                7215 bp ds-DNA     circular SYN 10-AUG-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7215)

  TITLE     Direct Submission

  JOURNAL   Exported Sep 15, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..7215

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             4..113

                     /codon_start=1

                     /label=Lip A SP

                     /translation="ILIWSASAAASKRMSMT*ISLLF*FLLNKRKS*HRR"

     primer_bind     complement(185..209)

                     /label=PMA0911R

     rep_origin      537..992

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        1018..1122

                     /gene="bla"

                     /label=AmpR promoter

     CDS             1123..1419

                     /codon_start=1

                     /label=Amp

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     DCNLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSAE"

     CDS             1466..1984

                     /codon_start=1

                     /label=Amp

                     /translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR

                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL

                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE

                     IGASLIKHW"

     rep_origin      2155..2746

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 

                     replication"

     promoter        complement(3516..3618)

                     /label=cat promoter

                     /note="promoter of the E. coli cat gene"

     CDS             4157..5161

                     /codon_start=1

                     /label=rep

                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY

                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI

                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV

                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN

                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS

                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"

     CDS             5321..6091

                     /codon_start=1

                     /label=Kana Neo

                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR

                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG

                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT

                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD

                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"

     CDS             6314..6712

                     /codon_start=1

                     /label=bleomycin

                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI

                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL

                     KDQWWDERDFAVIDPDNNLISFFQQIKS"

     primer_bind     7172..7195

                     /label=PMA0911F

ORIGIN

        1 catatcctca tatggtcagc atccgccgca gcttcgaagc gtatgtcgat gacatgaata

       61 tcattactgt tctgattcct gctgaacaaa aggaaatcat gacaccgccg gaattcgtcg

      121 acaagcttgg taccagatct gatatccctg cagggatcct ctagagtcga gctcaagcta

      181 gcttggtacg taccagatct gagatcccgg gttctagagg tcgaaattca cctcgaaagc

      241 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc

      301 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa

      361 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa

      421 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct

      481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc

      541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac

      601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt

      661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc

      721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc

      781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact

      841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg

      901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc

      961 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc

     1021 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

     1081 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc

     1141 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa

     1201 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgac

     1261 tgcaatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg

     1321 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa

     1381 gagcaactcg gtcgccgcat acactattct gcagaatgac ttggttgagt actcaccagt

     1441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac

     1501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct

     1561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga

     1621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac

     1681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat

     1741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg

     1801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc

     1861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc

     1921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg

     1981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta

     2041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg

     2101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga

     2161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt

     2221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag

     2281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa

     2341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag

     2401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca

     2461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac

     2521 cgaagtactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag

     2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct

     2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga

     2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc

     2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt

     2821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg

     2881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg

     2941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc

     3001 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc

     3061 accccaggct ttacacttta tgcttccggc atattctcaa taaacccttt agggaaatag

     3121 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa

     3181 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg

     3241 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat

     3301 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc

     3361 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctaaacggt ctggttatag

     3421 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata

     3481 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat

     3541 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa

     3601 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta

     3661 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat

     3721 tcgaagacga aagggcatcg cgcgcgggaa attcccggga gagctcgata tcgcatgcgg

     3781 tacctctaga agaagcttgg agacaaggta aaggataaaa cagcacaatt ccaagaaaaa

     3841 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa

     3901 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc

     3961 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat

     4021 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt

     4081 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgactaa

     4141 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta

     4201 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg

     4261 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa

     4321 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac

     4381 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa

     4441 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa

     4501 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg

     4561 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata

     4621 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa

     4681 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt

     4741 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat

     4801 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa

     4861 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt

     4921 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca

     4981 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct

     5041 tgatgacaca gaagaaggcg atttga