• 规格：20μl质粒
• 质粒大小：3760bp
• 原核抗性：氨苄青霉素Amp
• 质粒简介：       ATU6-26-sgRNA-SK质粒
• 质粒图谱1： png/1-1G01011005L63.png

质粒序列： 点击查看序列

LOCUS       Exported                3760 bp ds-DNA     circular SYN 26-SEP-2017

DEFINITION  synthetic circular DNA

KEYWORDS    ATU6-26-sgRNA-SK

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 3760)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 26, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..3760

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        64..168

                     /gene="bla"

                     /label=AmpR promoter

     CDS             169..1029

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1200..1789

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     misc_feature    2383..2389

                     /label=U6 terminator

     misc_RNA        complement(2390..2465)

                     /label=gRNA scaffold

                     /note="guide RNA scaffold for the Streptococcus pyogenes 

                     CRISPR/Cas9 system"

     misc_feature    complement(2483..2931)

                     /label=AtU6-26 promoter

                     /note="Synthetic construct guide RNA, complete sequence

                     GenBank: KT827739.1"

     rep_origin      complement(3176..3631)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

ORIGIN

        1 ctgatgtcgc gcgcttatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat

       61 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

      121 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

      181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

      241 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

      301 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

      361 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

      421 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

      481 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

      541 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

      601 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

      661 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

      721 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

      781 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

      841 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

      901 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

      961 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

     1021 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

     1081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

     1141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

     1201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

     1261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

     1321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc

     1381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

     1441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

     1501 gcgcagcggt cgggctgaac gggggggttc gtgcacacag cccagcttgg agcgaacgac

     1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg

     1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga

     1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact

     1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa

     1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc

     1861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg

     1921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat

     1981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt

     2041 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta

     2101 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg

     2161 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc

     2221 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg

     2281 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagcggcc

     2341 gccagtgtga tggatatctg cagaattgcc cttcgaaggg acaaaaaaag caccgactcg

     2401 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct

     2461 aaaacaggtc ttctcgaaga cccaatcact acttcgactc tagctgtata taaactcagc

     2521 ttcgttttct tatctaagcg atgtgggact tttgaagatt gttttcaact taaatgggcc

     2581 tatataagaa atactattgt tctttcccat ataaatgggc ctgcttctct tctttcagat

     2641 tcccaggggc cttttgaaga ttatcttcat atcttaagaa tgaagatgtt ttattcaatc

     2701 aaattcttga aggttcgatg cctaatcatt ctaatcctgg gacaaactat gaaacaagat

     2761 acaaaaactc cgaatggaaa gttaaaaaga agaaaacgaa agctacggtt caagaaaatg

     2821 taagctgata aacaaaaaaa aactgtatga acgaagaaga agaaaaaaag ctaagaagaa

     2881 atgatgtatt gtgcggaagg caagtcgagt ttccgttgtt caacgaagct taagggcaat

     2941 tccagcacac tggcggccgt tactagtgga tccgagctcg gtacccaatt cgccctatag

     3001 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg

     3061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga

     3121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt

     3181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt

     3241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg

     3301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc

     3361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca

     3421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga

     3481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa

     3541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc

     3601 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc

     3661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

     3721 taacccctga taaatgcttc aataatatga aaaagtaaga

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