- 商品货号:FH1384
- 规格:10μl质粒
- 启动子:T7 promoter
- 复制子:Ori,F1 ori
- 终止子:rrnB T1 terminator
- 质粒分类:大肠杆菌质粒;大肠其它质粒;其它大肠质粒
- 质粒大小:6495bp
- 质粒标签:SNAP-tag
- 原核抗性:氨苄青霉素Amp
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pSNAP-Tag(T7)2是一个大肠杆菌表达质粒,T7启动子驱动SNAP标签和目的基因融合表达。pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag® protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag(T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the Nor C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6 - alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAPtag substrates.
- 质粒图谱1: png/1-1FQG5215c41.png
质粒序列: 点击查看序列LOCUS Exported 6495 bp ds-DNA circular SYN 17-七月-2017KEYWORDS pSNAP-tag(T7)2SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 6495)AUTHORS MiaoLingBioFEATURES Location/Qualifierssource 1..6495/organism="synthetic DNA construct"/mol_type="other DNA"promoter 35..139/gene="bla"/note="AmpR promoter"CDS 140..1000/codon_start=1/gene="bla"/product="beta-lactamase"/note="AmpR"/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin complement(1100..1555)/direction=LEFT/note="F1 ori"/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"rep_origin 1666..2254/direction=RIGHT/note="ori"/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2623..2814)/codon_start=1/gene="rop"/product="Rop protein, which maintains plasmids at low copynumber"/note="rop"/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL"CDS complement(3371..4453)/codon_start=1/gene="lacI"/product="lac repressor"/note="lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"promoter complement(4454..4531)/gene="lacI"/note="lacI promoter"/note=""terminator 4684..4770/gene="Escherichia coli rrnB"/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"terminator 4867..4953/gene="Escherichia coli rrnB"/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"terminator 5050..5136/gene="Escherichia coli rrnB"/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"terminator 5233..5319/gene="Escherichia coli rrnB"/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"terminator 5416..5502/gene="Escherichia coli rrnB"/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"promoter 5637..5655/note="T7 promoter"/note="promoter for bacteriophage T7 RNA polymerase"protein_bind 5656..5680/bound_moiety="lac repressor encoded by lacI"/note="lac operator"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."CDS 5767..6312/codon_start=1/product="human O6-alkylguanine-DNA-alkyltransferasevariant that forms covalent bonds with benzylguaninederivatives"/note="SNAP-tag(R)"/note="optimized for bacterial expression"/translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLAVKEWLLAHEGHRLGKPGLG"terminator 6423..6470/note="T7 terminator"/note="transcription terminator for bacteriophage T7 RNApolymerase"ORIGIN1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat301 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag服务时间:24小时在线客服
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