• 规格：20μl质粒
• 复制子：pUC ori，F1 ori
• 质粒分类：基因文库质粒；其它基因质粒；其它种源质粒
• 质粒大小：4678bp
• 质粒标签：Kana-RpsL
• 原核抗性：氨苄青霉素Amp（100μg/ml）
• 克隆菌株：DH5α等大肠杆菌
• 表达宿主：293T等哺乳细胞
• 诱导方式：无需诱导
• 质粒简介：        pSK+KanaRpsL质粒了一个完整的KanaRpsL的基因模板。
• 质粒图谱1： png/1-1FQ4163023F1.png

质粒序列： 点击查看序列

LOCUS       Exported                4678 bp ds-DNA     circular SYN 14-AUG-2017

DEFINITION  synthetic circular DNA

KEYWORDS    pSK+KanaRpsL.dna

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4678)

  TITLE     Direct Submission

  JOURNAL   Exported Aug 14, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4678

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      complement(3..458)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        626..644

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             1031..1405

                     /codon_start=1

                     /label=RpsL

                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP

                     NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC

                     SGVKDRKQARSKYGVKRPKA"

     CDS             1446..2240

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     promoter        complement(2487..2505)

                     /label=T3 promoter

                     /note="promoter for bacteriophage T3 RNA polymerase"

     rep_origin      complement(2928..3516)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3687..4547)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4548..4652)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc

       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga

      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc

      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc

      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag

      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa

      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac

      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct

      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa

      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg

      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg

      661 gccccccctc gaggtcgacg gtatcgataa gcttcacgct gccgcaagca ctcagggcgc

      721 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc

      781 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag

      841 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca

      901 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta

      961 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctaaccag

     1021 gagctattta atggcaacag ttaaccagct ggtacgcaaa ccacgtgctc gcaaagttgc

     1081 gaaaagcaac gtgcctgcgc tggaagcatg cccgcaaaaa cgtggcgtat gtactcgtgt

     1141 atatactacc actcctaaaa aaccgaactc cgcgctgcgt aaagtatgcc gtgttcgtct

     1201 gactaacggt ttcgaagtga cttcctacat cggtggtgaa ggtcacaacc tgcaggagca

     1261 ctccgtgatc ctgatccgtg gcggtcgtgt taaagacctc ccgggtgttc gttaccacac

     1321 cgtacgtggt gcgcttgact gctccggcgt taaagaccgt aagcaggctc gttccaagta

     1381 tggcgtgaag cgtcctaagg cttaatggta gatctgatca agagacagga tgacggtcgt

     1441 ttcgcatgct tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc

     1501 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc

     1561 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg

     1621 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag

     1681 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg

     1741 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg

     1801 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac

     1861 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg

     1921 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc

     1981 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg

     2041 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc

     2101 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc

     2161 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc

     2221 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc

     2281 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg

     2341 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt

     2401 cttcgcccac cccgggctcg atcccctcgg ggggatccac tagttctaga gcggccgcca

     2461 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa

     2521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata

     2581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta

     2641 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa

     2701 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg

     2761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag

     2821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa

     2881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc

     2941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca

     3001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg

     3061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct

     3121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt

     3181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag

     3241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc

     3301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac

     3361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga

     3421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc

     3481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg

     3541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca

     3601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt

     3661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca

     3721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg

     3781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca

     3841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt

     3901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt

     3961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca

     4021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca

     4081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga

     4141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact

     4201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga

     4261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg

     4321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc

     4381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga

     4441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat

     4501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt

     4561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt

     4621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg

//