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- 规格：20μl质粒
- 启动子：Tac/lac
- 复制子：ColE1 ori
- 质粒分类：大肠杆菌载体；PGEX系列表达质粒
- 质粒大小：4983bp
- 质粒标签：N-GST，N-PP/3C
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：DH5α
- 表达宿主：大肠杆菌
- 诱导方式：IPTG或乳糖及其类似物
- 5测序引物：pGEX5: GGGCTGGCAAGCCACGTTTGGTG
- 3测序引物：pGEX3: CCGGGAGCTGCATGTGTCAGAGG
- 备注：可用GST亲和柱纯化重组蛋白
- 质粒简介： There are a range of Gluthatione Sepharose prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers.
- 质粒图谱1： gif/3-160Z41QP35C.gif
质粒序列： 点击查看序列
