• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：绿色荧光蛋白ZsGreen1
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：              pLVML-ECMV-Socs3-m(NM_007707.3) 是一个小鼠ORF基因慢病毒哺乳细胞表达质粒。
• 质粒图谱1： png/1-1P120160631442.png

质粒序列： 点击查看序列

LOCUS       Exported                8868 bp ds-DNA     circular SYN 23-AUG-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..8868

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     LTR             1..634

                     /label=3' LTR

                     /note="3' long terminal repeat (LTR) from HIV-1"

     misc_feature    681..806

                     /label=HIV-1 Psi

                     /note="packaging signal of human immunodeficiency virus 

                     type 1"

     misc_feature    1303..1536

                     /label=RRE

                     /note="The Rev response element (RRE) of HIV-1 allows for 

                     Rev-dependent mRNA export from the nucleus to the 

                     cytoplasm."

     misc_feature    2028..2143

                     /label=cPPT/CTS

                     /note="central polypurine tract and central termination 

                     sequence of HIV-1 (lacking the first T)"

     enhancer        2201..2504

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        2505..2708

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             2821..3498

                     /codon_start=1

                     /label=Socs3-m

                     /translation="MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESG

                     FYWSAVTGGEANLLLSAEPAGTFLIRDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSL

                     QSDPRSTQPVPRFDCVLKLVHHYMPPPGTPSFSLPPTEPSSEVPEQPPAQALPGSTPKR

                     AYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYD

                     APL"

     misc_feature    3507..4079

                     /label=IRES

                     /note="internal ribosome entry site (IRES) of the 

                     encephalomyocarditis virus (EMCV)"

     CDS             4081..4776

                     /codon_start=1

                     /product="Zoanthus green fluorescent protein"

                     /label=ZsGreen1

                     /note="mammalian codon-optimized"

                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN

                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV

                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG

                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH

                     AIASGSALP"

     misc_feature    4790..5378

                     /label=WPRE

                     /note="woodchuck hepatitis virus posttranscriptional 

                     regulatory element"

     LTR             5585..6218

                     /label=3' LTR

                     /note="3' long terminal repeat (LTR) from HIV-1"

     rep_origin      complement(6748..7336)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7507..8367)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(8368..8472)

                     /gene="bla"

                     /label=AmpR promoter

     polyA_signal    8520..8654

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

ORIGIN

        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca

       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac

      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca

      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg

      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag

      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg

      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat

      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga

      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct

      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc

      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag

      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg

      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga

      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg

      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg

      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct

      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat

     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca

     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc

     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag

     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc

     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg

     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc

     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc

     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct

     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa

     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca

     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt

     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt

     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta

     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt

     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct

     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga

     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag

     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac

     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg

     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa

     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg

     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt

     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg

     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc

     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc

     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca

     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta

     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa

     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc

     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc

     2821 atggtcaccc acagcaagtt tcccgccgcc gggatgagcc gccccctgga caccagcctg

     2881 cgcctcaaga ccttcagctc caaaagcgag taccagctgg tggtgaacgc cgtgcgcaag

     2941 ctgcaggaga gcggattcta ctggagcgcc gtgaccggcg gcgaggcgaa cctgctgctc

     3001 agcgccgagc ccgcgggcac ctttcttatc cgcgacagct cggaccagcg ccacttcttc

     3061 acgttgagcg tcaagaccca gtcggggacc aagaacctac gcatccagtg tgaggggggc

     3121 agcttttcgc tgcagagtga cccccgaagc acgcagccag ttccccgctt cgactgtgta

     3181 ctcaagctgg tgcaccacta catgccgcct ccagggaccc cctccttttc tttgccaccc

     3241 acggaaccct cgtccgaagt tccggagcag ccacctgccc aggcactccc cgggagtacc

     3301 cccaagagag cttactacat ctattctggg ggcgagaaga ttccgctggt actgagccga

     3361 cctctctcct ccaacgtggc caccctccag catctttgtc ggaagactgt caacggccac

     3421 ctggactcct atgagaaagt gacccagctg cctggaccca ttcgggagtt cctggatcag

     3481 tatgatgctc cactttaagg atcccgcccc tctccctccc ccccccctaa cgttactggc

     3541 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg

     3601 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct

     3661 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca

     3721 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg

     3781 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct

     3841 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa

     3901 tggctcacct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt

     3961 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa

     4021 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat

     4081 atggcccagt ccaagcacgg cctgaccaag gagatgacca tgaagtaccg catggagggc

     4141 tgcgtggacg gccacaagtt cgtgatcacc ggcgagggca tcggctaccc cttcaagggc

     4201 aagcaggcca tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc cgaggacatc