• 商品货号：FH1269

• 规格：10μl质粒
• 启动子：GAL1,GAL10 promoter
• 质粒大小：6706bp
• 筛选标记：DH5α
• 克隆菌株：37℃，有氧，LB
• 质粒简介：          The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.  These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters.  Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone.            The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies.   A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies.            • Express two different genes simultaneously in S. cerevisiae 
             • Proteins tagged with unique epitope tags 
             • Fast and easy immunoprecipitations
• 质粒图谱1： png/1-1FR31A626260.png

质粒序列： 点击查看序列

LOCUS       Exported                6706 bp ds-DNA     circular SYN 13-APR-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pESC-HIS

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 6706)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..6706

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        317..503

                     /gene="S. cerevisiae HIS3"

                     /note="HIS3 promoter"

     CDS             504..1163

                     /codon_start=1

                     /gene="S. cerevisiae HIS3"

                     /product="imidazoleglycerol-phosphate dehydratase, required

                     for histidine biosynthesis"

                     /note="HIS3"

                     /note="yeast auxotrophic marker"

                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE

                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF

                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL

                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"

     rep_origin      complement(1423..1878)

                     /direction=LEFT

                     /note="f1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     terminator      1961..2126

                     /gene="S. cerevisiae ADH1"

                     /note="ADH1 terminator"

                     /note="transcription terminator for the S. cerevisiae 

                     alcohol dehydrogenase 1 (ADH1) gene"

     CDS             complement(2274..2297)

                     /codon_start=1

                     /product="FLAG(R) epitope tag, followed by an enterokinase 

                     cleavage site"

                     /note="FLAG"

                     /translation="DYKDDDDK"

     promoter        2344..3008

                     /gene="GAL1 and GAL10 genes of S. cerevisiae"

                     /note="GAL1,10 promoter"

                     /note="divergent inducible promoter, regulated by Gal4"

     protein_bind    2560..2677

                     /bound_moiety="Gal4"

                     /note="UAS"

                     /note="upstream activating sequence mediating 

                     Gal4-dependent induction"

     promoter        3019..3037

                     /note="T7 promoter"

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             3055..3084

                     /codon_start=1

                     /product="Myc (human c-Myc oncogene) epitope tag"

                     /note="Myc"

                     /translation="EQKLISEEDL"

     terminator      3114..3303

                     /gene="S. cerevisiae CYC1"

                     /note="CYC1 terminator"

                     /note="transcription terminator for CYC1"

     rep_origin      complement(3546..4134)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4305..5165)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5166..5270)

                     /gene="bla"

                     /note="AmpR promoter"

     rep_origin      5297..6639

                     /note="2u ori"

                     /note="yeast 2u plasmid origin of replication"

     protein_bind    complement(6260..6307)

                     /bound_moiety="FLP recombinase from the Saccharomyces 

                     cerevisiae 2u plasmid"

                     /note="FRT"

                     /note="FLP-mediated recombination occurs in the 8-bp core 

                     sequence TCTAGAAA (Turan and Bode, 2011)."

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt

      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta

      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat

      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata

      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc

      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa

      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact

      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga

      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt

      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca

      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag

      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag

      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag

      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta

     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca

     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct

     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat

     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat

     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt

     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt

     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata

     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg

     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc

     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa

     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt

     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac

     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta

     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg

     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc

     1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc

     1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa

     2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata

     2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg

     2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg

     2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg

     2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg

     2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt

     2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat

     2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt

     2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg

     2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt

     2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact

     2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat

     2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct

     2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa

     2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca

     2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga

     3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag

     3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct

     3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata

     3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga

     3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg

     3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg

     3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg

     3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga