- 商品货号:FH1201
- 规格:10μl质粒
- 质粒图谱1: png/1-1F92Q32Z4292.png
质粒序列: 点击查看序列LOCUS Exported 6547 bp ds-DNA circular SYN 12-JUN-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS pCDNA3.1-3×FLAG-RBM4SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 6547)AUTHORS .TITLE Direct SubmissionJOURNAL Exported Sep 28, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..6547/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 235..614/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 615..818/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 863..881/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"primer_bind 863..881/label=T7CDS 922..987/codon_start=1/product="three tandem FLAG(R) epitope tags, followed by anenterokinase cleavage site"/label=3xFLAG/translation="DYKDHDGDYKDHDIDYKDDDDK"primer_bind 1002..1026/label=GC87-FCDS 1009..2103/codon_start=1/product="Homo sapiens RNA binding motif protein 4, mRNA(cDNA clone MGC:45319IMAGE:5540605), complete cdsGenBank: BC032735.1"/label=RBM4/translation="MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKTSTKLHVGNISPTCTNKELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMHVQLSTSRLRTAPGMGDQSGCYRCGKEGHWSKECPIDRSGRVADLTEQYNEQYGAVRTPYTMSYGDSLYYNNAYGALDAYYKRCRAARSYEAVAAAAASVYNYAEQTLSQLPQVQNTAMASHLTSTSLDPYDRHLLPTSGAAATAAAAAAAAAAVTAASTSYYGRDRSPLRRATAPVPTVGEGYGYGHESELSQASAAARNSLYDMARYEREQYADRARYSAF"primer_bind complement(2084..2109)/label=GC87-Rprimer_bind complement(2141..2158)/label=BGHpolyA_signal 2147..2371/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 2417..2845/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 2859..3188/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 3039..3174/label=SV40 ori/note="SV40 origin of replication"CDS 3255..4049/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 4223..4344/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"primer_bind complement(4393..4409)/label=M13 rev/note="common sequencing primer, one of multiple similarvariants"protein_bind 4417..4433/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."promoter complement(4441..4471)/label=lac promoter/note="promoter for the E. coli lac operon"protein_bind 4486..4507/label=CAP binding site/bound_moiety="E. coli catabolite activator protein"/note="CAP binding activates transcription in the presenceof cAMP."rep_origin complement(4795..5380)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(5551..6411)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(6412..6516)/gene="bla"/label=AmpR promoterORIGIN1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccat ggtgaagctg1021 ttcatcggaa acctgccccg ggaggctaca gagcaggaga ttcgctcact cttcgagcag1081 tatgggaagg tgctggaatg tgacatcatt aagaattacg gctttgtgca catagaagac1141 aagacggcag ctgaggatgc catacgcaac ctgcaccatt acaagcttca tggggtgaac1201 atcaacgtgg aagccagcaa gaataagagc aaaacctcaa caaagttgca tgtgggcaac1261 atcagtccca cctgcaccaa taaggagctt cgagccaagt ttgaggagta tggtccggtc1321 atcgaatgtg acatcgtgaa agattatgcc ttcgtacaca tggagcgggc agaggatgca1381 gtggaggcca tcaggggcct tgataacaca gagtttcaag gcaaacgaat gcacgtgcag1441 ttgtccacca gccggcttag gactgcgccc gggatgggag accagagcgg ctgctatcgg1501 tgcgggaaag aggggcactg gtccaaagag tgtccgatag atcgttcagg ccgcgtggca1561 gacttgaccg agcaatataa tgagcaatac ggagcagtgc gtacgcctta caccatgagc1621 tatggggatt cattgtatta caacaacgcg tacggagcgc tcgatgccta ctacaagcgc1681 tgccgtgctg cccggtccta tgaggcagtg gcagctgcag ctgcctccgt gtataattac1741 gcagagcaga ccctgtccca gctgccacaa gtccagaata cagccatggc cagtcacctc1801 ac
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