• 商品货号：FH1016

• 规格：10μl质粒
• 复制子：pUC
• 质粒大小：3851bp
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：            pDrive是一个基因克隆质粒。
• 质粒图谱1： png/3-16102G61543549.png

质粒序列： 点击查看序列

LOCUS       Exported                3851 bp ds-DNA     circular SYN 09-一月-2013
DEFINITION  Bacterial vector for UA cloning and expression of a PCR product.
KEYWORDS    pDrive
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Qiagen
  TITLE     Direct Submission
  JOURNAL   Exported 2016年9月18日 from SnapGene Viewer 2.8.1
            http://www.mialingbio.com
COMMENT     Linearize at EcoRV to create UA cloning vector.
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        142..172
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             216..596
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSSNTTHYRESSVPRMLQTRYVSDPEFVISEFVDKLLEPR
                     LALDHTCGGPSSRPLYSIVSPKWPHNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEARTDRPSQQLRSLNGEWKL"
     promoter        239..257
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    266..393
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(399..417)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(431..447)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(588..1043)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1070..1174
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1175..2035
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             2181..2996
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
      241 atacgactca ctatagggaa agctcggtac cacgcatgct gcagacgcgt tacgtatcgg
      301 atccagaatt cgtgatatct gaattcgtcg acaagcttct cgagcctagg ctagctctag
      361 accacacgtg tgggggcccg agctcgcggc cgctgtattc tatagtgtca cctaaatggc
      421 cgcacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      481 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      541 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
      601 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
      661 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      721 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      781 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
      841 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
      901 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
      961 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     1021 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1141 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2101 gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct gcttacataa
     2161 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc
     2221 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     2281 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     2341 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     2401 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     2461 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     2521 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     2581 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     2641 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     2701 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     2761 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     2821 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     2881 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     2941 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat
     3001 taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3841 gaagcggaag a
//