- 商品货号:FH1827
- 规格:10μl质粒
- 原核抗性:卡那霉素Kan
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: 参考文献:CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
- 质粒图谱1: png/1-1P12119535AR.png
质粒序列: 点击查看序列LOCUS Exported 11982 bp ds-DNA circular SYN 21-JAN-2018FEATURES Location/Qualifierssource 1..11982/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin 2672..2894/label=pSC101 ori/note="low-copy replication origin that requires the Rep101protein"CDS 2942..3892/codon_start=1/gene="rep101"/product="RepA protein needed for replication with thepSC101 origin"/label=Rep101/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"terminator 6149..6234/gene="Escherichia coli rrnB"/label=rrnB T1 terminator/note="transcription terminator T1 from the E. coli rrnBgene"terminator 6326..6353/label=rrnB T2 terminator/note="transcription terminator T2 from the E. coli rrnBgene"CDS complement(6397..10299)/codon_start=1/label=Cpf1/translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNN"CDS complement(10392..11186)/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Kan/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"ORIGIN1 tcctcacgta gtgccaaagg aacgcgtgcg gtgaccacga cgggcttagc ctttgcctgc61 gcttctagtg cttcgatggt ggcttgtgcc tgcgcttgct gcgcctgtag tgcctgttga121 gcttcttgta gttgctgttc tagctgtgcc ttggttgcca tgctttaaga ctctagtagc181 tttcctgcga tatgtcatgc gcatgcgtag caaacattgt cctgcaactc attcattatg241 tgcagtgctc ctgttactag tcgtacatac tcatatttac ctagtctgca tgcagtgcat301 gcacatgcag tcatgtcgtg ctaatgtgta aaacatgtac atgcagattg ctgggggtgc361 agggggcgga gccaccctgt ccatgcgggg tgtggggctt gccccgccgg tacagacagt421 gagcaccggg gcacctagtc gcggataccc cccctaggta tcggacacgt aaccctccca481 tgtcgatgca aatctttaac attgagtacg ggtaagctgg cacgcatagc caagctaggc541 ggccaccaaa caccactaaa aattaatagt ccctagacaa gacaaacccc cgtgcgagct601 accaactcat atgcacgggg gccacataac ccgaaggggt ttcaattgac aaccatagca661 ctagctaaga caacgggcac aacacccgca caaactcgca ctgcgcaacc ccgcacaaca721 tcgggtctag gtaacactga aatagaagtg aacacctcta aggaaccgca ggtcaatgag781 ggttctaagg tcactcgcgc tagggcgtgg cgtaggcaaa acgtcatgta caagatcacc841 aatagtaagg ctctggcggg gtgccatagg tggcgcaggg acgaagctgt tgcggtgtcc901 tggtcgtcta acggtgcttc gcagtttgag ggtctgcaaa actctcactc tcgctggggg961 tcatctctgg ctgaattgga agtcatgggc gaacgccgca ttgagctggc tattgctact1021 aagaatcact tggcggcggg tggcgcgctc atgatgtttg tgggcactgt tcgacacaac1081 cgctcacagt catttgcgca ggttgaagcg ggtattaaga ctgcgtactc ttcgatggtg1141 aaaacatctc agtggaagaa agaacgtgca cggtacgggg tggagcacac ctatagtgac1201 tatgaggtca cagactcttg ggcgaacggt tggcacttgc accgcaacat gctgttgttc1261 ttggatcgtc cactgtctga cgatgaactc aaggcgtttg aggattccat gttttcccgc1321 tggtctgctg gtgtggttaa ggccggtatg gacgcgccac tgcgtgagca cggggtcaaa1381 cttgatcagg tgtctacctg gggtggagac gctgcgaaaa tggcaaccta cctcgctaag1441 ggcatgtctc aggaactgac tggctccgct actaaaaccg cgtctaaggg gtcgtacacg1501 ccgtttcaga tgttggatat gttggccgat caaagcgacg ccggcgagga tatggacgct1561 gttttggtgg ctcggtggcg tgagtatgag gttggttcta aaaacctgcg ttcgtcctgg1621 tcacgtgggg ctaagcgtgc tttgggcatt gattacatag acgctgatgt acgtcgtgaa1681 atggaagaag aactgtacaa gctcgccggt ctggaagcac cggaacgggt cgaatcaacc1741 cgcgttgctg ttgctttggt gaagcccgat gattggaaac tgattcagtc tgatttcgcg1801 gttaggcagt acgttctcga ttgcgtggat aaggctaagg acgtggccgc tgcgcaacgt1861 gtcgctaatg aggtgctggc aagtctgggt gtggattcca ccccgtgcat gatcgttatg1921 gatgatgtgg acttggacgc ggttctgcct actcatgggg acgctactaa gcgtgatctg1981 aatgcggcgg tgttcgcggg taatgagcag actattcttc gcacccacta aaagcggcat2041 aaaccccgtt cgatattttg tgcgatgaat ttatggtcaa tgtcgcgggg gcaaactatg2101 atgggtcttg ttgttggcgt cccggagcgt aaaggatcta ggtgaagatc ctttttgata2161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccttgatg2221 ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg gataatccga2281 agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag tcagatagca2341 ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt ttcttgtatt2401 atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt acccccattc2461 actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg actcaggtgc2521 ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga gggtgctact2581 taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt gcgacatcct2641 tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg gatctattct2701 ttttatcttt ttttattctt tctttattct ataaattata accacttgaa tataaacaaa2761 aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta caagttttcc2821 agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga ctagtaatta2881 tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc aattgagatg2941 tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct atgacttaac3001 ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca cgattgaaaa3061 ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgctc agatgatgaa3121 catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc tgatgacgag3181 aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt ggacaaacta3241 tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat tgccttatct3301 tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt ttgaaaacaa3361 atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga aaactcacaa3421 ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg aaaataacta3481 ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag atttaaacac3541 ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata cgttgatttt3601 ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca accagataaa3661 aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac ataacggact3721 aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa3781 atttttgagt gacatgcaaa gtaagcatga tctcaatggt tcgttctcat ggctcacgca3841 aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct gaggttctta3901 tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc3961 accgggcccg tgggtggcta ggcaagttac agaactgatc ggataaaagc agagttatat4021 ctgatgaatt gctattagca gtatcgttat cacagcacca acaaagtagt tcagccacag4081 gaaaactttc caactgcgat tagcctgttc acaactggca tctgtaatgt tccaaaatcg4141 tgcggcatta aatacgtaag ttagaatcgc aatcccgatg atccacgccg gattaggcaa4201 agtagtgact aacacagcag ctagtaaata aagtactact gaaagccgaa tggctccacg4261 cgccccaatt acagtggcaa ttgagctcag attagcttcc cggtctgcat taacatcctg4321 tactgctcca aggatctgac tggccatgcc ccacaagaaa aaggatccca gtgctatcca4381 catcgctgct gaaggagatg ttccagtgat cgttgcaccg attaatgcag gtgaagtgaa4441 gtgagtagaa gatgttagag catcgataaa ggggcgttct ttaaaacgca atttcggtgc4501 tgaataagca atcactgcta gcactgagag tgtcagccat aaagacgaca tccaggtgcc4561 aaatatgaaa agaataacta ggaaaggaat tgttgagata gccgaggccc ataacagtgt4621 gctgtgggaa cttttcggta gcacggcccc ctcgacgccg cctttgcggg gattacgcat4681 atcagattcg taatcaaaaa catcgttgat accatacatg gcgatgttat acgggataag4741 aaaaaatacg atgcctagcc aaaacagcca gtcaatctct cctgcattta ataggtaggc4801 cagaccaaag gggtaggcgg tattgatcca gctaatgggg cgagatgaca atagaattag4861 tcttattttt tccatcatga ctacggcttt tctggctc
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