• 商品货号：FH1806

• 规格：10μl质粒
• 启动子：35S
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌HB101
• 质粒简介：        pRI201-ON是一个单子叶植物发根农杆菌的双表达质粒。The Ri Plasmid pRI201 is designed for the transformation and expression of target genes from plant cells. This series of binary vectors for plant transformation retains the backbone of the pRI101 vectors, including an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5'-UTR) downstream of the cauliflower mosaic virus (CaMV promoter)-derived 35S promoter. Additionally, these vectors have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI101 vector series. Multigene transformation with a single vector can be achieved by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the vector's second multiple cloning site (MCS2) located downstream of the HSP terminator.
          The Ri Plasmid pRI201 binary vector series offers two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. pRI 201-AN DNA contains an Arabidopsis ADH-derived 5' UTR (AtADH 5'-UTR) and is suitable for dicotyledonous plant transformation. pRI 201-ON DNA contains a rice ADH-derived 5' UTR (OsADH 5'-UTR) and is suitable for monocotyledonous plant transformation. The pRI 201-AN DNA and pRI 201-ON DNA binary vectors for plant transformation have a mutant-type replication origin (Ri ori) from the Rhizobium rhizogenes Ri plasmid. These vectors also contain a replication origin (ColE1 ori) derived from the pUC plasmid; the ColE1 ori allows high copy number replication in E. coli. The vectors' multiple cloning site, located near the right border (RB) of T-DNA, allows stable target gene integration into the plant chromosome.
• 质粒图谱1： gif/1-1P106120534945.gif

质粒序列： 点击查看序列

LOCUS       Exported               10475 bp ds-DNA     circular SYN 23-NOV-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..10475

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      complement(57..645)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6419..7213)

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin)"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     promoter        7620..7650

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    7658..7674

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     primer_bind     7682..7698

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     primer_bind     complement(9075..9091)

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar 

                     variants"

ORIGIN

        1 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc

       61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga

      121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct

      181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg

      241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag

      301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat

      361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac

      421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac

      481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc

      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt

      601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc

      661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg

      721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca

      781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca

      841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcga tcctacaagg

      901 tagaatccgc ctgagtcgca agggtgactt cgcctatatt ggacgacggc gcgcagaggg

      961 cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg aacatattca

     1021 aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt ggagtttttc

     1081 gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag cagaatcggc

     1141 gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa atatcgccac tggcaaggaa

     1201 tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag ggttgacggc

     1261 cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc atcttaaggt

     1321 tttcccgccg cattccgaga agggtattcg aacattcggg ccatcggagg cgtccaagct

     1381 gctcggcgtt ggcgagtcat atttacggca gaccgcgtct gagatgccag agttgaatgt

     1441 tagcatgagc ccaggtggca ggcgaatgtt ctcaattgaa gatatccatg tgattcggaa

     1501 gtatatggat caggtcggcc gcgggaaccg gcgctacctg ccacatcgtc gaggcggcga

     1561 gcagcttcag gttatctctg tgatgaattt caaaggtggg tcgggtaaga ccaccaccgc

     1621 cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga gtcttggcca ttgatctcga

     1681 tcctcaagcg agcctttctg cactctttgg gagccaaccg gagacggacg ttggcccgaa

     1741 cgaaacgctc tacggcgcta taaggtatga tgatgagcag gtggcaatcg aacgagtcgt

     1801 ccgagggact tacattcccg acctccacct gattcctggt aaccttgagc tgatggagtt

     1861 tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag ggcgacacgc tcttttatgg

     1921 tcgcatcagc caagtaattg aagatatcgc ggataactat gacgtcgtgg tcatcgactg

     1981 ccctccccag cttgggtatc tcacgctatc cgcattgact gcggcgacgt ccattcttgt

     2041 cacggtccat ccgcagatgc tggatgtgat gtcgatgaac cagtttctgg caatgacatc

     2101 gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc aagtttaatt ggatgcgcta

     2161 tctgataacc cgtttcgaac cgagcgacgg accacagaac caaatggtag gttatctgcg

     2221 gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt aaaaccaccg cggtttcgga

     2281 cgctggcctg acaaaccaga ctctattcga agtggagcgt ggcctgttca cgcgctcgac

     2341 ctatgatcga gccttggagg cgatgaacgc cgtcaacgac gagatcgaaa cactgatcaa

     2401 aaaagcatgg ggtaggccca catgagccgg aagcacatcc ttggcgtctc aactgacgcc

     2461 cctgagacgt cgcccgccga caataggacg gcaaagaacc gctccatgcc gctcctcggc

     2521 gtaacaagga aggagcgcga tccggcaacg aagctcacag cgaacattgg taacgcactg

     2581 cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg agcggcgtct cgctgaaggt

     2641 caggcagtga tagagttgga tgcctcgtca atagaaccgt ctttcgtgca ggatcgtatg

     2701 cgaggggaca ttgacgggct ccttacttcg atccgggaac aaggacagca agtcccaatc

     2761 cttgtgcgac cgcatccgag ccagccgggc cgatatcagg ttgccttcgg ccaccgccgg

     2821 ctacgcgccg tttcagaact cggacttccg gtcagggcgg tcgttcgcga actgacggac

     2881 gagcaagtgg tcgtagcaca gggtcaggaa aacaatgtgc gcgaagatct taccttcatc

     2941 gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt ctcgagagat tgtcatcgcc

     3001 gcgatgtcga tcgacaagag caatttgtcc aagatgcttc tgctcgttga cgccctcccc

     3061 tctgaactga ccgatgctat tggtgccgct cctggtgttg gacggccgag ttggcaacaa

     3121 cttgccgagc tgattgagaa agtttcttca ccggccgacg tggctaaata tgctatgtcg

     3181 gaggaagttc aagcgctgcc atcggcagaa cgattcaagg cggtgatcgc tagtctgaag

     3241 cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca ccccagacgg caccagaatt

     3301 gcacaggtga cgcagagcaa ggccaaactg gaaatcacga ttgacaggaa ggcgacgccc

     3361 gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt atcaagcgta ccacgctgag

     3421 aaccaacgga aacggggaga gtaaaccgca aaagaaaaga gccccctcaa cgtcgccgtc

     3481 gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca tttcctcgaa tcctcgtcaa

     3541 gagtttttag cgccgttttg gtgagctgat ttcctttgcc tgctgaaagg tgaaagatga

     3601 tgcagacagg aagtgtaacg acgccattcg ggcggcggcc aatgacgctt gcgcttgtgc

     3661 ggcgccagac ggcgctggcc gatatcaaac aaggcaagac agcggacaag tggaaggtct

     3721 ttagggacgc gtccgcggcc atggaactac ttggaatcca gtccaacagt cttgccgtcc

     3781 ttgatgcgct attgagcttt cacccggaaa cggagttgcg tcaggaggca cagctgatcg

     3841 tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg gatggctggc gcgactttgc

     3901 gtaggcacat cgccatgctc gtggagtcag gcttgatcgt ccggaaggat agcgccaacg

     3961 gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga gcgcgcgttt ggcttcgatt

     4021 tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat ggcacagcag gtgatggccg

     4081 atcgagcagc attcaggatg gccaaagaaa gtctgacgat ttgccgacgg gacgttcgga

     4141 agctaattac ggcagctatg gaagagggag cggagggcga ctggcaagct gtcgaggaag

     4201 tctatgtgga acttgtgggt agaatcccac gcgccccgac gcttgctgat gtagagtcaa

     4261 ttctcgaaga gatgtggatg ctccaggaag agataatcaa ccggttggaa attagagaca

     4321 attcagaaaa taatagcacc aatgctgccc agagcgagca gcacatacag aactcaaaac

     4381 ccgaatccgt taatgaactt gaacctcgct ctgaaaagga gcagggcgct aagccgagtg

     4441 aaatagaccg ggcaaggagc gagccgataa aagcgttccc cctcgggatg atcctgaaag

     4501 cgtgcccgac cattggcaat tatgggccga gcggtgcggt tgctagctgg cgtgacctca

     4561 tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag cccgtcggct taccaagacg

     4621 cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat ggcgtgcatt ttggagcgag

     4681 cgaacttcat caattcgcct gggggctatc tccgagatct gacacggcgg agcgaactcg

     4741 ggaagttttc acttggcccg atgataatgg cgctcttgaa ggctagcggg caggggacgt

     4801 tgcggtttgg ctagaattag cgagtatgga gcaggatggt ctgtggtcag ctgaccacag

     4861 acctaatagg ttgaaaacat gagcgttttt tggatgatcg acagaccatc cgattcccgg

     4921 agtaccaagc gtgctctgat gggagcgata acattactca acaagcacga aggccccatg

     4981 ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg cggtattttg ccggccgagg

     5041 catgtagtcg cggagcactg cctatttact gccctaggca caaacgttga ctcttggatc

     5101 gagctggcag acaaagcaat aacccacaca gaggacgatt aatggctgac gaagagatcc

     5161 agaatccgcc ggacggtact gctgctgccg aagttgagcc ggctgctcct agaggtagaa

     5221 gagcaaagaa agcaccagcc gaaacagccc gcacgggatc gttcaaatcc gtgaagccga

     5281 aaacccgcgg cctcagcaac cgagaaaaac tggagaagat cggtcaaatc gaagctcagg

     5341 tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt gggtatttcc gttcagacct

     5401 attatcaatg gaagagagct gcggttcaac ctgtctcaca gaatccggcc gtgtctgttt

     5461 cagttgacga tgaactcggc gagttcatcc aactcgagga ggaaaatcgg cggctcagaa

     5521 agctagcttg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata

     5581 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc

     5641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg

     5701 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt

     5761 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc

     5821 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg

     5881 gcccaaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg

     5941 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa

     6001 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat

     6061 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac

     6121 tttattgcca aatgtttgaa cgatcggggc ccgatcatat tgtcgctcag gatcgtggcg

     6181 ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca

     6241 gagatcccgt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg

     6301 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa

     6361 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc

     6421 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac

     6481 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg

     6541 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc

     6601 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga

     6661 cgagatcatc gccgtcgggc atacgcgcct tgagcctggc gaacagttcg gctggcgcga

     6721 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac

     6781 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg

     6841 tatgcag