• 规格：10μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：URA3
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：             pCU-MET3-GFP是一个念珠菌绿色荧光表达质粒。
• 质粒图谱1： png/1-1G2191GQ0N6.png

质粒序列： 点击查看序列

LOCUS       Exported                7009 bp ds-DNA     circular SYN 19-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..7009

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        666..737

                     /gene="bla"

                     /label=AmpR promoter

     CDS             738..1598

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1769..2357

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     misc_feature    2817..3842

                     /label=MET3 promoter

     CDS             3879..4595

                     /codon_start=1

                     /product="yeast-enhanced green fluorescent protein"

                     /label=yeGFP

                     /note="codons optimized for translation in S. cerevisiae 

                     and C. albicans"

                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK

                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG

                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV

                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE

                     FVTAAGITHGMDELYK"

     rep_origin      5211..5666

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     CDS             complement(5797..6600)

                     /codon_start=1

                     /gene="S. cerevisiae URA3"

                     /product="orotidine-5'-phosphate decarboxylase, required 

                     for uracil biosynthesis"

                     /label=URA3

                     /note="yeast auxotrophic marker, counterselectable with 

                     5-fluoroorotic acid (5-FOA)"

                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL

                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ

                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE

                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD

                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

     promoter        complement(6601..6816)

                     /gene="S. cerevisiae URA3"

                     /label=URA3 promoter

ORIGIN

        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

       61 cttagcaggt tagactaaca tgcaaaaagt ttatattcat ttttgtagtt attcttgttt

      121 tcattaggat gattacacac tgtggatgtg gattaatcga tcattgttaa tttagtgtag

      181 ctactaccat ttcaaacaaa acgtaaattc ttgcagtcgt actggatctg tgaatctatt

      241 agtatatatg aattaaagta gcttgtacat attattctgt tgaatcatat cgcagagcat

      301 tggttgaaat ccaaaatata aaaatgtaat atcacaaaaa ataatactaa ttctaacatt

      361 aatgggtcag atttttagtg aatacttaaa tttataatat cgtctattta agctagcaaa

      421 tggaacaaca tttaaagtaa gaacatcata tctacatgaa aatgtatatt tcaatctgac

      481 taataacgca gagcacatct ttcagtgatg tctgtcacat gatcaaaaag aattgtattt

      541 aaataattca taataaaagc ttaaaaaatt acaataatga aaataaagta aataatgaca

      601 tgggtaagag ttccgaaata gaatcttagt gtacaaaaca aaaattgcat cattagagat

      661 ccccacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat

      721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg

      781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg

      841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc

      901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat

      961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact

     1021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca

     1081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact

     1141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg

     1201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg

     1261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg

     1321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg

     1381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag

     1441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc

     1501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga

     1561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat

     1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc

     1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag

     1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct

     1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac

     1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc

     1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg

     1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt

     2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt

     2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc

     2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca

     2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata

     2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg

     2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct

     2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta

     2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag

     2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga

     2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg

     2641 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg

     2701 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc

     2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcatac

     2821 cagttacaat tagtattaca atggtttacc aagtacaaat tatgtaatat ggttataatt

     2881 ggcttatatg tatggaggta gggaactgca ggttattgtt ctgttaaact accagtaatg

     2941 caagacggct aagtcatatg actgccactt tgtcaatcct gaagaaaatg acaatagaaa

     3001 gtgatataat acacgtgata tgcgtaggat gggacccaaa gatagccaca tcacatgatc

     3061 ttcaaaaacc ccgtcaaaaa ggaataaatc gaaggaaaaa aaatgccacg tgactttgat

     3121 agtcaaaatc aggttatatt actatacgat cccccacaca taacctttac cacacaccgc

     3181 acgggctgga cttatcctat tagaaagcgg tgcagccagg gccaagaaaa cgcgaacgac

     3241 gcagaaaaaa cgtcaagcaa aaaaactgtg gtgttattta aagcatacaa tttcctgcct

     3301 ccctttaatg ctacggggga tctagcaaat gggaaaatat cacatgactt tggctagaag

     3361 gggtgggaaa acagatattt tagtcacatt gtttgatttc acgtactaca cgatacacta

     3421 cactacacaa tacactgcaa tacactacac tgtatggttc cctccggccc tggatatgct

     3481 agcgaaggat cccaagccca tcgaggaaat cattcaagag catctgcagg tctcaaatac

     3541 actaagtcca aacacaaaca gcactgagct aatccaagac accaattgca tgccttctca

     3601 attagaacag tttattacac agcttataac tgtgcatctt gccattcttt tcgagatagc

     3661 catttgcatt tgattaacat gcttagctcg ttctcaagcc acagtaaaat ggattgcctt

     3721 ttgagtttcc atcgttgtat aaataggcac tcattcctat agccttgctc tttggtcttg

     3781 tctctgtaat ggtaatagct gtaagtcagg atataacact ccagaaaaga aacacctaac

     3841 aatctagaac tagtggatcc cccgggctgc aggaattcat gtctaaaggt gaagaattat

     3901 tcactggtgt tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt

     3961 ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaatttattt

     4021 gtactactgg taaattgccg gttccatggc caaccttagt cactactttc ggttatggtg

     4081 ttcaatgttt tgctagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca

     4141 tgccagaagg ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga

     4201 ccagagctga agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta

     4261 ttgattttaa agaagatggt aacattttag gtcacaaatt ggaatacaac tataactctc

     4321 acaatgttta catcatggct gacaaacaaa agaatggtat caaagttaac ttcaaaatta

     4381 gacacaacat tgaagatggt tctgttcaat tagctgacca ttatcaacaa aatactccaa

     4441 ttggtgatgg tccagtcttg ttaccagaca accattactt atccactcaa tctgccttat

     4501 ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt agaatttgtt actgctgctg

     4561 gtattaccca tggtatggat gaattgtaca aataactgca ggtcgacctc gagaacacaa

     4621 cccacagcta ccaccatcaa caatatttat atatataacg tacacataga aatcacacaa

     4681 acagagtatt tattcttaac tacatgaact accatcagac cgtctgggcc actatataat

     4741 gtgccattca taaacgtgat cactttacgt agcaggcaac cccaggtgaa aatttttcag

     4801 cgagctgcca gattgtcagg tgaaaaactg aaaaaaactt ctgggcgatg agcttgtggc

     4861 gggaaattaa gtatataaag cagttagttt cttctgcttc ttgtggttct gggtattctt

     4921 gtaatatttg gaagaataga aaatcaagac tactgccttt cttttcatat tatagaggaa

     4981 gaaatacgca cgaacacgat atagaggtaa aggtacccaa ttcgccctat agtgagtcgt

     5041 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta

     5101 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg

     5161 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg

     5221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc

     5281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg

     5341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg

     5401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg

     5461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt

     5521 ccaaactgga acaacactc