• 商品货号：FH1711

• 规格：10μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：荧光素酶Luc
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：             p53-Luc是一个哺乳细胞单荧光素信号通路报告质粒。
• 质粒图谱1： png/1-1G2201F602b8.png

质粒序列： 点击查看序列

LOCUS       Exported                5914 bp ds-DNA     circular SYN 07-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5914

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        32..136

                     /gene="bla"

                     /label=AmpR promoter

     CDS             137..997

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     misc_feature    1934..2074

                     /label=bom

                     /note="basis of mobility region from pBR322"

     polyA_signal    2313..2447

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     polyA_signal    2541..2673

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     CDS             3030..4682

                     /codon_start=1

                     /gene="luc"

                     /product="firefly luciferase"

                     /label=luciferase

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"

     intron          4813..4878

                     /label=small t intron

                     /note="SV40 (simian virus 40) small t antigen intron"

     CDS             5008..5028

                     /codon_start=1

                     /product="nuclear localization signal of SV40 (simian virus

                     40) large T antigen"

                     /label=SV40 NLS

                     /translation="PKKKRKV"

     polyA_signal    5453..5587

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     RBS             5622..5630

                     /label=Shine-Dalgarno sequence

                     /note="full consensus sequence for ribosome-binding sites 

                     upstream of start codons in E. coli; complementary to a 

                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"

ORIGIN

        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta

       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata

      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc

      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga

      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct

      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg

      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta

      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat

      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt

      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga

      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga

      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga

      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc

      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc

      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg

      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat

      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata

     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct

     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga

     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg

     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc

     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct

     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc

     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt

     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg

     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca

     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag

     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag

     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg

     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc

     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc

     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg

     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc

     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac

     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga

     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc

     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca

     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga

     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt

     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta

     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcagc catatcacat

     2461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata

     2521 aaatgaatgc aattgttgtt aacttgttta ttgcagctta taatggttac aaataaagca

     2581 atagcatcac aaatttcaca aataaagcaa tttttcactg cattctattg tggtttgtcc

     2641 aaactcatca atgtatctta tcatgtctgg atccaagctc ctgcctggac ttgcctggcc

     2701 tgcctggact tgcctggcct gcctggactt gcctgcctgg acttgcctgg cctgcctgga

     2761 cttgcctggc ctgcctggac ttgcctggcc tgcctggact tgcctggcct gcctggactt

     2821 gcctggcctg cctggacttg cctggcctgc ctggacttgc ctggcctgcc tggacttgcc

     2881 tggcctgcct ggacttgcct ggcctgcctg gacttgcctg gcctgcctgg acttgcctgg

     2941 cctgcctgga cttgcctggg cggaggactc tagaggggta tataatggaa gctcgaattc

     3001 cagcttggca ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc

     3061 cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga

     3121 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca

     3181 tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg

     3241 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc

     3301 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg

     3361 aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa

     3421 aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta

     3481 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc

     3541 atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa

     3601 caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc

     3661 cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa

     3721 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta

     3781 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag

     3841 agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc

     3901 tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg

     3961 aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct

     4021 tccatcttcc agggatacga caaggatatg ggctcactga gactacatca gctattctga

     4081 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag

     4141 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat

     4201 gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct

     4261 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac

     4321 acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc

     4381 ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag

     4441 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa

     4501 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa

     4561 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg

     4621 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt

     4681 aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta ttgtaatact ctagaggatc

     4741 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt

     4801 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta

     4861 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat

     4921 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct

     4981 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag

     5041 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt

     5101 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg

     5161 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt

     5221 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt

     5281 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg

     5341 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct

     5401 cccacacctc cc