- 商品货号:FH1663
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:LEU2
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: pJH001是一个酵母CAS9表达基因敲除质粒。
- 质粒图谱1: png/1-1G203122212I9.png
质粒序列: 点击查看序列LOCUS Exported 11928 bp ds-DNA circular SYN 03-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..11928/organism="synthetic DNA construct"/mol_type="other DNA"CDS complement(664..1758)/codon_start=1/gene="S. cerevisiae LEU2"/product="3-isopropylmalate dehydrogenase, required forleucine biosynthesis"/label=LEU2/note="yeast auxotrophic marker"/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA"promoter complement(1759..2166)/gene="S. cerevisiae LEU2"/label=LEU2 promoterrep_origin complement(2467..2922)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 3194..3860/gene="S. cerevisiae TDH3"/label=GAP promoter/note="promoter for glyceraldehyde-3-phosphatedehydrogenase"regulatory 3889..3898/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"CDS 3895..7998/codon_start=1/product="Cas9 (Csn1) endonuclease from the Streptococcuspyogenes Type II CRISPR/Cas system"/label=Cas9/note="generates RNA-guided double strand breaks in DNA"/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"CDS 8008..8028/codon_start=1/product="nuclear localization signal of SV40 (simian virus40) large T antigen"/label=SV40 NLS/translation="PKKKRKV"terminator 8056..8243/gene="S. cerevisiae ADH1"/label=ADH1 terminator/note="transcription terminator for the S. cerevisiaealcohol dehydrogenase 1 (ADH1) gene"rep_origin complement(8764..9352)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(9523..10383)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(10384..10488)/gene="bla"/label=AmpR promoterrep_origin 10515..11861/label=2u ori/note="yeast 2u plasmid origin of replication"ORIGIN1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga841 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc1081 agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc1141 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac1501 actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt1561 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatctatag cagcaccacc1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat1741 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga1801 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa1861 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg1921 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc1981 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt2041 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga2101 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc2161 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca2221 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac2281 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt2341 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct2401 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc2461 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat&nbs
服务时间:24小时在线客服
客服热线:400-000-7085
丰晖生物
中国优质生物资源平台