- 商品货号:ZT566
- 规格:10μl质粒
- 启动子:CaMV 35S promoter
- 终止子:NOS terminator
- 质粒大小:10832bp
- 质粒标签:N-HA
- 原核抗性:卡那霉素Kan
- 筛选标记:潮霉素Hyg
- 克隆菌株:大肠杆菌DB3.1
- 质粒简介: pCXSN-HA质粒是一个植物过表达质粒,35S启动子驱动HA和目的基因融合表达,在ccdb致死基因的作用下,克隆基因变得更加容易,阳性率高。
- 质粒图谱1: png/1-1G0301P355205.png
质粒序列: 点击查看序列LOCUS Exported 10832 bp ds-DNA circular SYN 30-OCT-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 10832)TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..10832/organism="synthetic DNA construct"/mol_type="other DNA"terminator 7..270/label=NOS terminator/note="nopaline synthase terminator and poly(A) signal"CDS 627..932/codon_start=1/gene="ccdB"/product="CcdB, a bacterial toxin that poisons DNA gyrase"/label=ccdB/note="Plasmids containing the ccdB gene cannot bepropagated in standard E. coli strains."/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"CDS complement(988..1014)/codon_start=1/product="HA (human influenza hemagglutinin) epitope tag"/label=HA/translation="YPYDVPDYA"promoter complement(1032..1922)/label=CaMV 35S promoter/note="strong constitutive promoter from cauliflower mosaicvirus"misc_feature 2156..2180/label=RB T-DNA repeat/note="right border repeat from nopaline C58 T-DNA"CDS 3479..4108/codon_start=1/product="stability protein from the Pseudomonas plasmidpVS1 (Heeb et al., 2000)"/label=pVS1 StaA/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"CDS 4542..5609/codon_start=1/product="replication protein from the Pseudomonas plasmidpVS1 (Heeb et al., 2000)"/label=pVS1 RepA/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"rep_origin 5675..5869/label=pVS1 oriV/note="origin of replication for the Pseudomonas plasmidpVS1 (Heeb et al., 2000)"misc_feature 6213..6353/label=bom/note="basis of mobility region from pBR322"rep_origin complement(6539..7127)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(7214..8008)/codon_start=1/gene="aphA-3"/product="aminoglycoside phosphotransferase"/label=KanR/note="confers resistance to kanamycin"/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"misc_feature 8433..8457/label=LB T-DNA repeat/note="left border repeat from nopaline C58 T-DNA"polyA_signal 8535..8709/label=CaMV poly(A) signal/note="cauliflower mosaic virus polyadenylation signal"CDS complement(8749..9774)/codon_start=1/gene="aph(4)-Ia"/product="aminoglycoside phosphotransferase from E. coli"/label=HygR/note="confers resistance to hygromycin"/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKK"promoter complement(9841..10518)/label=CaMV 35S promoter (enhanced)/note="cauliflower mosaic virus 35S promoter with aduplicated enhancer region"ORIGIN1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt961 ctgcaggtcg acccatacaa gtattggagc gtaatctgga acatcgtatg ggtacatgga1021 tccccctcga gtatcgttcg taaatggtga aaattttcag aaaattgctt ttgctttaaa1081 agaaatgatt taaattgctg caatagaagt agaatgcttg attgcttgag attcgtttgt1141 tttgtatatg ttgtgttgag gtcgaggtcc tctccaaatg aaatgaactt ccttatatag1201 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat1261 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc1321 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct1381 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa1441 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt1501 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga1561 caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg1621 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt1681 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc1741 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga1801 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga1861 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca1921 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg1981 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag2041 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca2101 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca2161 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa2221 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc2281 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg2341 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca2401 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta2461 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct2521 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact2581 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg2641 cccggagctg gccaggatgc ttgaccacct agccctggcg acgttgtgac agtgaccagg2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac2881 cgcacccgga gcgggcgcga ggccgccaag gcccga
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