• 商品货号：FH1603

• 规格：10μl质粒
• 启动子：T7/lac promoter
• 终止子：T7 terminator
• 质粒大小：5634bp
• 质粒标签：N-Strep-Tag II，N-HRV 3C site
• 原核抗性：壮观霉素Spe
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：              pWUR702质粒可诱导表达TtAgo蛋白，在N端携带有Strep-Tag II标签和HRV 3C site蛋白酶酶切位点。本质粒参考文献为：DNA-guided DNA interference by a prokaryotic Argonaute. 
• 质粒图谱1： png/1-1G025161K5921.png

质粒序列： 点击查看序列

LOCUS       Exported                5634 bp ds-DNA     circular SYN 25-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5634)

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5634

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     protein_bind    3..27

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     CDS             80..103

                     /codon_start=1

                     /product="peptide that binds Strep-Tactin(R), an engineered

                     form of streptavidin"

                     /label=Strep-Tag II

                     /translation="WSHPQFEK"

     CDS             110..133

                     /codon_start=1

                     /product="recognition and cleavage site for human 

                     rhinovirus 3C and PreScission proteases"

                     /label=HRV 3C site

                     /translation="LEVLFQGP"

     misc_feature    141..2200

                     /label=TtAgo

                     /note="Thermus thermophilus   TTHB068    AP008227 "

     terminator      2315..2362

                     /label=T7 terminator

                     /note="transcription terminator for bacteriophage T7 RNA 

                     polymerase"

     CDS             complement(2533..3324)

                     /codon_start=1

                     /gene="aadA"

                     /product="aminoglycoside adenylyltransferase (Murphy, 

                     1985)"

                     /label=Spe

                     /note="confers resistance to spectinomycin and 

                     streptomycin"

                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH

                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK

                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF

                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP

                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"

     promoter        complement(3325..3416)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      complement(3464..4202)

                     /direction=LEFT

                     /label=CloDF13 ori

                     /note="Plasmids containing the CloDF13 (CDF) origin of 

                     replication can be propagated in E. coli cells that contain

                     additional plasmids with compatible origins."

     CDS             complement(4412..5494)

                     /codon_start=1

                     /gene="lacI"

                     /product="lac repressor"

                     /label=lacI

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL

                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV

                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH

                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA

                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC

                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR

                     ALADSLMQLARQVSRLESGQ"

     promoter        complement(5495..5572)

                     /gene="lacI"

                     /label=lacI promoter

     misc_feature    5618..2

                     /label=T7 promoter

ORIGIN

        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag

       61 gagatatacc atgtcaagct ggagccaccc gcagttcgaa aagggtgcac ttgaagtcct

      121 ctttcaggga cccgggtacc agatgaacca ccttggaaaa acggaagtgt tcctaaaccg

      181 cttcgcccta cggcctctga accccgagga actcaggcct tggcgcctcg aggtggttct

      241 ggaccctcct ccggggcggg aggaggtgta tccccttctc gcccaggtgg cccgccgggc

      301 ggggggcgtc acggtgcgca tgggagacgg cctcgcctcc tggtcacccc ctgaggtcct

      361 ggtcttggag ggcaccttgg cgcggatggg gcagaactac gcctaccgcc tctaccccaa

      421 ggggaggagg cctctggacc ccaaggaccc gggggagcgg agcgtgcttt cggccctagc

      481 ccgaaggctc cttcaggagc gcctcaggcg cctcgagggg gtctgggtgg aggggcttgc

      541 ggtgtaccgg agggagcacg cccgggggcc cgggtggcgg gtgcttgggg gggcggtctt

      601 ggacctttgg gtctccgact cgggggcgtt cctcctggag gtggaccccg cttaccgcat

      661 cctctgcgag atgtccctcg aggcctggct tgcccaaggc caccctctgc ccaaacgggt

      721 ccggaacgcc tacgaccggc gcacctggga gcttctccgg ctgggagagg aagaccccaa

      781 ggagctccct ctcccgggcg gcttgagcct cctggactac cacgctttca agggccgcct

      841 ccagggccgg gaaggggggc gggtggcctg ggtggcggac ccaaaggatc cgcgaaagcc

      901 aatcccccat ctgacgggcc ttttggtgcc cgttctgacc ctggaagacc ttcatgaaga

      961 ggagggaagc ctggccctct ccttgccttg ggaagagcgg cgtcggcgga cccgggagat

     1021 cgccagctgg atcggccggc gcctggggct gggcacacct gaggcggtgc gcgcccaggc

     1081 ttaccgcttg agcatcccca agctcatggg cagaagagcc gtgagcaagc ctgcggacgc

     1141 cctccgcgtg gggttttacc gggcccagga aaccgccctg gccctcttgc gcctggacgg

     1201 ggcccaagga tggccggagt ttctccggcg ggccttgctc cgggcctttg gggcgagcgg

     1261 ggcttccctc cgtttgcaca ccctccacgc ccatccctcc caaggcctgg ctttccgcga

     1321 ggcgctgcgg aaggccaagg aggagggggt ccaggccgtg ctggtcctca ccccgcccat

     1381 ggcctgggaa gaccgcaacc gcctgaaggc ccttcttctc agggaaggcc ttcccagcca

     1441 aatcctcaac gtccccctcc gggaggagga acgccaccgc tgggagaacg ccctcctggg

     1501 cctcctggcc aaagcggggc ttcaggtggt ggccctgagc ggcgcctatc cggcggagct

     1561 cgccgtgggc tttgacgccg gcggaaggga gtcctttcgc ttcgggggcg cggcctgcgc

     1621 cgtgggcggg gacggcggcc acctcctctg gaccctcccc gaggcccagg ccggggagcg

     1681 gatcccccag gaggtggtct gggacctctt ggaggagacc ctctgggcct tcagacgcaa

     1741 ggcggggagg cttccttccc gggtcctcct ccttcgggac ggccgcgtgc cccaggacga

     1801 gttcgccctg gccttggagg cccttgcccg ggaaggcata gcctacgact tggtttcggt

     1861 gcgcaagtcg ggtggggggc gggtctaccc cgtgcagggg cgcctggcgg acgggcttta

     1921 cgtccccttg gaggacaaga cctttcttct ccttaccgtc caccgggact tccggggcac

     1981 gccccgaccc ctgaagctgg tgcacgaggc gggggacacg cccctcgagg ccctggccca

     2041 ccagattttc catctgaccc gcctctaccc ggcgagcggt ttcgccttcc cccggcttcc

     2101 cgctcccctt cacctggccg accgcctggt gaaggaggtg ggccggttgg ggatccgtca

     2161 cctcaaggag gtggaccggg aaaagctctt cttcgtttag gaattcgcgg ccgcagagct

     2221 cgctctggtg ccacgcggta gttccgctca tcaccaccat catcaccatc accaccacta

     2281 attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa

     2341 acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact

     2401 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc

     2461 ctgccctgaa ccgacgaccg ggtcatcgtg gccggatctt gcggcccctc ggcttgaacg

     2521 aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa

     2581 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg

     2641 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc

     2701 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt

     2761 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt

     2821 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc

     2881 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat

     2941 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa

     3001 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt

     3061 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc

     3121 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc

     3181 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag

     3241 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac

     3301 ttcggcgatc accgcttccc tcatactctt cctttttcaa tattattgaa gcatttatca

     3361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagc

     3421 tagctcactc ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc ggacacatac

     3481 aaagttaccc acagattccg tggataagca ggggactaac atgtgaggca aaacagcagg

     3541 gccgcgccgg tggcgttttt ccataggctc cgccctcctg ccagagttca cataaacaga

     3601 cgcttttccg gtgcatctgt gggagccgtg aggctcaacc atgaatctga cagtacgggc

     3661 gaaacccgac aggacttaaa gatccccacc gtttccggcg ggtcgctccc tcttgcgctc

     3721 tcctgttccg accctgccgt ttaccggata cctgttccgc ctttctccct tacgggaagt

     3781 gtggcgcttt ctcatagctc acacactggt atctcggctc ggtgtaggtc gttcgctcca

     3841 agctgggctg taagcaagaa ctccccgttc agcccgactg ctgcgcctta tccggtaact

     3901 gttcacttga gtccaacccg gaaaagcacg gtaaaacgcc actggcagca gccattggta

     3961 actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc

     4021 aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa gccagttacc

     4081 acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc caggtggttt

     4141 tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa gatcctttga

     4201 tcttttctac tgaaccgctc tagatttcag tgcaatttat ctcttcaaat gtagcacctg

     4261 aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg

     4321 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg