细胞信息
发货规格	1×10⁶
别名	T 98 G; T-98G; T98 G; T98-G
形态	成纤维细胞样
生长特性	贴壁
培养条件	MEM+10%FBS+1%P/S
冻存条件	90%FBS+10%DMSO
传代方式	胰酶消化
倍增时间	28~40h
传代比例	1:2~1:4
换液频率	每周2~3次
生物安全等级	1
细胞背景
种属	人源
组织来源	脑
肿瘤类型	胶质母细胞瘤
供体信息	61岁男性欧洲人
背景描述	T98G [T98-G] 是一种成纤维细胞样细胞，是从多形性胶质母细胞瘤 White 的大脑中分离出来的
遗传背景
核型	This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?)， i(6p)， der(10)t(10;?) (q24;?)， der (19)t(19;?) (q13;?)， der(15)t(15;?) (q26?;?)， minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation， i.e.， t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5， N7， N11， N13， N20， N21， and N22 in most cells. The X and N15 had only one copy.

STR鉴定

STR位点信息

STR鉴定图

Amelogenin	X,Y
CSF1PO	10,12
D2S1338	19,24
D3S1358	16
D5S818	10,12
D7S820	9,10
D8S1179	13,14
D13S317	13
D16S539	13
D18S51	13,16
D19S433	12,13
D21S11	28,32.2
FGA	21 (PubMed=22570425; PubMed=25877200) 21,22 (ATCC=CRL-1690; COG; KCLB=21690)
Penta D	10,11
Penta E	16
TH01	7,9.3
TPOX	8
vWA	17,20