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| 发货规格 |
1×10⁶ |
| 别名 |
HUC-1; SV-HUC; SVHUC |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
F12K+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
输尿管、尿路上皮 |
| 背景描述 |
SV-HUC-1 是从一名 11 岁男性的尿路上皮细胞中分离出来的上皮细胞。该系是通过用 SV40 病毒转化正常输尿管组织建立的。该细胞已使用非洲绿猴肾细胞斑块测定法反复测试了传染性 SV40 的生产，并且始终呈阴性。压力（如化学品暴露）可能会激活病毒。 |
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| 蛋白表达 |
Uroepithelial Keratins， Sv40 T Antigen |
| 致瘤性 |
no |
| 核型 |
The chromosome number distribution is bimodal with 50% of the cells near diploid (mode = 44 chromosomes) and 50% tetraploid (mode = 88).Both populations consistently showed seven marker chromosomes: 5p+， del(6)(p11)， 9q+， 11p+， 15q-， 19p+ and Xp+. Most cells lacked a normal chromosome 15.Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21， while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation. |
