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发货规格 |
1×10⁶ |
别名 |
H358; H-358; NCIH358 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
RPMI-1640+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
38~60h |
传代比例 |
1:3~1:6 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
肺 |
肿瘤类型 |
非小细胞肺癌 |
背景描述 |
该系由A.F. Gazdar,H.K. Oie,J.D. Minna及其同事于1981年从患者开始化疗前获得的肿瘤组织中得出。超微结构研究表明,存在 Clara 细胞特征的细胞质结构。 |
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基因表达 |
lung surfactant associated protein A (SP-A); protein and RNA of SP-A, the major lung surfactant associated protein |
致瘤性 |
裸鼠 |
核型 |
modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells. |