• 靶点-Target： FEN-1
• MW(Observed)： 45kD
• 宿主物种-Host Species： Mouse
• 修饰-Modification： Unmodified
• 推荐稀释比： WB 1:1000
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 克隆性： Monoclonal
• 免疫原： Purified recombinant human FEN-1 protein fragments expressed in E.coli.
• 特异性： This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.
• 基因名称： fen1
• Organism-1： Human
• 基因ID-1： 2237
• SwissProt-1： P39748
• Organism-2： Mouse
• SwissProt-2： P39749
• 背景： The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate， thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore， secondary structure can deter the protective function of this protein， leading to site-specific trinucleotide expansions
• 细胞定位： [Isoform 1]: Nucleus， nucleolus. Nucleus， nucleoplasm. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion .

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